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Communication Dans Un Congrès Année : 2016

Evolution of urine metabolomic profiles in newborns

Résumé

Metabolomics provides untargeted identification of all detectable low molecular-weight molecules by profiling without a priori the metabolic signatures of biological samples in connection to pathophysiological events. The goal of metabolomic studies is to identify relevant biomarkers or composite metabolic patterns associated with particular disease status. Urine is particularly suited for metabolomic analysis in newborns and children, due to its simple and non-invasive method of collection. Its biochemical composition is correlated to a number of factors such as genotype, gender, disease, nutritional state and age. The number of metabolomic studies in pediatrics is rising, but little is known concerning age-related changes in urine metabolic profiles and newborn metabolic maturation over time. The aim of this study was to investigate changes in urine metabolic profiles during the first four months of life using 1H-nuclear magneticresonance (NMR) spectroscopy combined with multivariate statistical analysis. Urine samples were collected from 91 newborns under 4 months old without nephrologic or urologic disease. The mean age was 68 days ± 24. The1H-NMR spectra were analyzed using Principal Component Analysis (PCA) and the effect of age on the urinary metabolite profile was observed even from this unsupervised analysis. Further analysis using Orthogonal Partial Least Squares (OPLS) methodology was performed and a model with good predictive power was calculated, allowing the identification of an age-related metabolic profile. We observed the most significant evolution between 2 and 3 months of life. Our results allow a deeper understanding of newborn metabolic maturation. They contribute to identifying potential confounding factors in the application of metabolomics in newborns.
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Dates et versions

hal-01575756 , version 1 (21-08-2017)

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  • HAL Id : hal-01575756 , version 1

Citer

Aurélien Scalabre, Elodie Jobard, Ségolène Gaillard, Bénédicte Elena-Herrmann, Pierre-Yves Mure. Evolution of urine metabolomic profiles in newborns. 10èmes Journées Scientifiques du Réseau Francophone de Métabolomique et Fluxomique , RFMF - Réseau Francophone de Métabolomique et Fluxomique May 2016, Montpellier, France. ⟨hal-01575756⟩
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