Article Dans Une Revue Laser Physics Letters Année : 2010

Synchrotron based Fourier-transform infrared microspectroscopy as sensitive technique for the detection of early apoptosis in U-87 MG cells

Résumé

The aim of this study is to show that SR-FTIRM can be successfully applied for the detection of early apoptotic processes in the human glioma cells (U-87 MG). The apoptosis was induced by photodynamic action after irradiation of either photosensitizer hypericin (Hyp) or a complex of Hyp with low-density lipoproteins (Hyp/LDL) incorporated into the cells. The differences between infrared (IR) spectra of non-treated and photodynamically treated U-87 MG cells are mainly manifested in position of Amide I vibrational band, sensitive to changes in protein secondary structure. The conformational transition α-helix to β-sheet results in the shift of Amide I vibration to lower frequency, and can be explained as a consequence of the processes leading to apoptosis. It is also shown that the sensitivity of SR-FTIRM for the detection of early apoptotic signs is comparable, or even higher, than that of classic technique usually used for the detection of early apoptosis, flow-cytometry study of cell staining by Annexin V. Moreover, we have demonstrated by both methods, SR-FTIRM and flow-cytometry, that Hyp/LDL complex loaded U-87 MG cells 24 hours after photodynamic action are mostly late apoptotic/necrotic. In contrast, U-87 MG cells loaded with Hyp alone display apoptosis as prevailing mode of cell death at 4 hours and 24 hours after photodynamic action.

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hal-01570994 , version 1 (01-08-2017)

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L Buriankova, Z Nadova, J Jancura, Matthieu M. Refregiers, I Yousef, et al.. Synchrotron based Fourier-transform infrared microspectroscopy as sensitive technique for the detection of early apoptosis in U-87 MG cells. Laser Physics Letters, 2010, 7 (8), pp.613 - 620. ⟨10.1002/lapl.201010033⟩. ⟨hal-01570994⟩
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