Saccharification performances of Miscanthus at the pilot and miniaturized assay scales: genotype and year variabilities according to the biomass composition - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Frontiers in Plant Science Année : 2017

Saccharification performances of Miscanthus at the pilot and miniaturized assay scales: genotype and year variabilities according to the biomass composition

Résumé

The large genetic diversity of the perennial grass miscanthus makes it suitable for producing cellulosic ethanol in biorefineries. The saccharification potential and year variability of five genotypes belonging to Miscanthus x giganteus and Miscanthus sinensis were explored using a miniaturized and semi-automated method, allowing the application of a hot water treatment followed by an enzymatic hydrolysis. The studied genotypes highlighted distinct cellulose conversion yields due to their distinct cell wall compositions. An inter-year comparison revealed significant variations in the biomass productivity and cell wall compositions. Compared to the recalcitrant genotypes, more digestible genotypes contained higher amounts of hemicellulosic carbohydrates and lower amounts of cellulose and lignin. In contrast to hemicellulosic carbohydrates, the relationships analysis between the biomass traits and cellulose conversion clearly showed the same negative effect of cellulose and lignin on cellulose digestion. The miniaturized and semi-automated method we developed was usable at the laboratory scale and was reliable for mimicking the saccharification at the pilot scale using a steam explosion pretreatment and enzymatic hydrolysis. Therefore, this miniaturized method will allow the reliable screening of many genotypes for saccharification potential. These findings provide valuable information and tools for breeders to create genotypes combining high yield, suitable biomass composition, and high saccharification yields.
Fichier principal
Vignette du fichier
Belmokhtar etal,2017_Saccharification miscanthus_frontiers in Plant Science_{10A7E1A7-AD9F-42FB-9B33-CC90CE1ECE2C}.pdf (1.31 Mo) Télécharger le fichier
Belmokhtar etal,2017_Saccharification miscanthus_frontiers in Plant Science_{ECE668BC-D96B-4022-B5E3-746717150E9E}.pdf (1.31 Mo) Télécharger le fichier
Origine : Accord explicite pour ce dépôt
Origine : Accord explicite pour ce dépôt

Dates et versions

hal-01570309 , version 1 (28-07-2017)

Licence

Paternité - Partage selon les Conditions Initiales

Identifiants

Citer

Nassim Belmokhtar, Stéphanie Arnoult-Carrier, Brigitte Chabbert, Jean-Paul Charpentier, Maryse Brancourt. Saccharification performances of Miscanthus at the pilot and miniaturized assay scales: genotype and year variabilities according to the biomass composition. Frontiers in Plant Science, 2017, volume 8, pp.1-13. ⟨10.3389/fpls.2017.00740⟩. ⟨hal-01570309⟩
86 Consultations
101 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More