Bicelle membranes and their use for hydrophobic peptide studies by circular dichroism and solid state NMR - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biochimica et Biophysica Acta (BBA) - General Subjects Année : 2005

Bicelle membranes and their use for hydrophobic peptide studies by circular dichroism and solid state NMR

L. Khemtémourian
  • Fonction : Auteur
F. Aussenac
  • Fonction : Auteur
Stéphane Gineste
M.-F. Achard
  • Fonction : Auteur

Résumé

Mixtures of dicaproyl- (DC), dimyristoyl- (DM) and 1-tetradecanoyl-2- biphenylbutanoyl-(TBB) phosphatidylcholine (PC) in water produce bicelle membranes that are oriented by magnetic fields. DMPC/DCPC systems orient such that their membrane plane is parallel to the magnetic field, whereas for TBBPC/DCPC, the plane is perpendicular to the field. Partial temperature-composition-hydration diagrams are established using solid-state 31P-NMR. DMPC/DCPC bicelles exist on a large range of composition but on a narrow temperature domain (25-45°C). At converse, TBBPC/DCPC form bicelles on a narrow compositional range but over a large temperature span (10-70°C). The TBBPC/DCPC bicelles are shown to be a very powerful potential tool to study the orientation of hydrophobic helices in membranes using wide line 15N-NMR. The DMPC/DCPC system that undergoes a micelle-to-bicelle transition on going from 10°C to 40°C may be used with circular dichroism to study the state of association of hydrophobic helices within the membrane. Results suggest that the transmembrane fragment of the neu/erbB-2 receptor is monomeric in micellar medium and dimeric/multimeric in bicelle membranes. © 2005 Elsevier B.V. All rights reserved.

Dates et versions

hal-01564163 , version 1 (18-07-2017)

Identifiants

Citer

C. Loudet, L. Khemtémourian, F. Aussenac, Stéphane Gineste, M.-F. Achard, et al.. Bicelle membranes and their use for hydrophobic peptide studies by circular dichroism and solid state NMR. Biochimica et Biophysica Acta (BBA) - General Subjects, 2005, 1724 (3), pp.315-323. ⟨10.1016/j.bbagen.2005.04.026⟩. ⟨hal-01564163⟩

Collections

CNRS
28 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More