First landscape of binding to chromosomes for a domesticated mariner transposase in the human genome: diversity of genomic targets of SETMAR isoforms in two colorectal cell lines - Archive ouverte HAL Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2017

First landscape of binding to chromosomes for a domesticated mariner transposase in the human genome: diversity of genomic targets of SETMAR isoforms in two colorectal cell lines

Linda Beauclair
  • Fonction : Auteur
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Solenne Bire
Nicolas Buisine
Vincent Coustham
Yves Bigot

Résumé

Setmar is a 3-exons gene coding a SET domain fused to a Hsmar1 transposase. Its different transcripts theoretically encode 8 isoforms with SET moieties differently spliced. In vitro, the largest isoform binds specifically to Hsmar1 DNA ends and with no specificity to DNA when it is associated with hPso4. In colon cell lines, we found they bind specifically to two chromosomal targets depending probably on the isoform, Hsmar1 ends and sites with no conserved motifs. We also discovered that the isoforms profile was different between cell lines and patient tissues, suggesting the isoforms encoded by this gene in healthy cells and their functions are currently not investigated.

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Dates et versions

hal-01558001 , version 1 (06-07-2017)

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Aymeric Antoine-Lorquin, Ahmed Arnaoty, Sassan Asgari, Martine Batailler, Linda Beauclair, et al.. First landscape of binding to chromosomes for a domesticated mariner transposase in the human genome: diversity of genomic targets of SETMAR isoforms in two colorectal cell lines. 2017. ⟨hal-01558001⟩
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