Wireless and Portable System for the Study of in-vitro Cell Culture Impedance Spectrum by Electrical Impedance Spectroscopy
Résumé
A wireless and portable system, consisting in an
electronic board and a computer software graphical interface,
is presented in this article as a feasible way to do in-vitro
electric bioimpedance spectroscopy of cells. It is designed to
work inside a culture chamber performing impedance
measurement in the frequency range of 64Hz to 200KHz. The
board is equipped with a Bluetooth Low Energy (BLE) module
allowing it to be wirelessly controlled. The software interface
(also called ISMI) is coded with all required functionalities to
manage the parameters of the board such as start frequency
and sweep, measuring intervals, data acquisition and
visualization and also a function to perform automatic
measuring between desired time intervals during whole day for
many days. In addition, the electrodes used for the
measurements of cells are characterized giving an impedance
magnitude between 103 to 105 ohms in a frequency range of
300Hz to 100KHz and a maximum voltage without
considerable electrode deterioration of 120mVpeak. This
information is used in the calibration of the ISMI system giving
a measurement accuracy above 98% when compared with
simulation results and with a reference instrument. The
proposed system is an introductory step in the study of cells
related to fibrous tissue induced by implants showing to be a
viable and reproducibility-improving approach for such
analysis. This first prototype provides information regarding
the requirements for the design of an integrated version for
embedded applications.