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Communication Dans Un Congrès Année : 2017

Wireless and Portable System for the Study of in-vitro Cell Culture Impedance Spectrum by Electrical Impedance Spectroscopy

Résumé

A wireless and portable system, consisting in an electronic board and a computer software graphical interface, is presented in this article as a feasible way to do in-vitro electric bioimpedance spectroscopy of cells. It is designed to work inside a culture chamber performing impedance measurement in the frequency range of 64Hz to 200KHz. The board is equipped with a Bluetooth Low Energy (BLE) module allowing it to be wirelessly controlled. The software interface (also called ISMI) is coded with all required functionalities to manage the parameters of the board such as start frequency and sweep, measuring intervals, data acquisition and visualization and also a function to perform automatic measuring between desired time intervals during whole day for many days. In addition, the electrodes used for the measurements of cells are characterized giving an impedance magnitude between 103 to 105 ohms in a frequency range of 300Hz to 100KHz and a maximum voltage without considerable electrode deterioration of 120mVpeak. This information is used in the calibration of the ISMI system giving a measurement accuracy above 98% when compared with simulation results and with a reference instrument. The proposed system is an introductory step in the study of cells related to fibrous tissue induced by implants showing to be a viable and reproducibility-improving approach for such analysis. This first prototype provides information regarding the requirements for the design of an integrated version for embedded applications.
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Dates et versions

hal-01552359 , version 1 (02-07-2017)

Identifiants

  • HAL Id : hal-01552359 , version 1

Citer

Edwin de Roux, Mehdi Terosiet, Florian Kolbl, Michel Boissière, Philippe Banet, et al.. Wireless and Portable System for the Study of in-vitro Cell Culture Impedance Spectrum by Electrical Impedance Spectroscopy. Euromicro Conference on Digital System Design, Aug 2017, Vienna, Austria. ⟨hal-01552359⟩
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