Acute amnestic encephalopathy in amyloid-beta oligomer-injected mice is due to their widespread diffusion in vivo - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Neurobiology of Aging Année : 2015

Acute amnestic encephalopathy in amyloid-beta oligomer-injected mice is due to their widespread diffusion in vivo

Résumé

Amyloid-beta (A beta) oligomers are the suspected culprit as initiators of Alzheimer's disease (AD). However, their diffusion in the brain remains unknown. Here, we studied A beta oligomers' dissemination and evaluated their in vivo toxicity. Wild-type mice were injected with 50 pmol of synthetic A beta oligomers (of different size) in the hippocampus. Oligomers diffused largely in the brain as soon as 1 hour and up to 7 days after injection. A transient encephalopathy with memory impairment was induced by this unique injection. The immunoreactivity of the postsynaptic marker PSD95 was diffusely decreased. Similar results (both on memory and PSD95 immunoreactivity) were obtained with delipidated and high molecular weight oligomers (>50 kDa) but not with smaller assemblies. Tau hyperphosphorylation was observed in the oligomer-injected brains. Finally, fos immunostaining was increased in A beta-derived diffusible ligandseinjected mice, suggesting neuronal hyperactivity. Rapid and widespread diffusion of Ab oligomers was demonstrated in vivo and associated with decreased synaptic markers and memory deficits which gives new insight to the pathogenicity of A beta.
Fichier principal
Vignette du fichier
2015BDelatour_Acute amnestic encephalopathy.pdf (5.46 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-01544165 , version 1 (25-05-2021)

Identifiants

Citer

Stephane Epelbaum, Ihsen Youssef, Pascale N. Lacor, Pierre Chaurand, Eric Duplus, et al.. Acute amnestic encephalopathy in amyloid-beta oligomer-injected mice is due to their widespread diffusion in vivo. Neurobiology of Aging, 2015, 36 (6), pp.2043-2052. ⟨10.1016/j.neurobiolaging.2015.03.005⟩. ⟨hal-01544165⟩
70 Consultations
86 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More