TRANSPARENT TESTA 16 and 15 act through different mechanisms to control proanthocyanidin accumulation in Arabidopsis testa - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Experimental Botany Année : 2017

TRANSPARENT TESTA 16 and 15 act through different mechanisms to control proanthocyanidin accumulation in Arabidopsis testa

Wenjia Xu
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Sophie Bobet
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Damaris Grain
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Délphine de Vos
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Adeline Berger
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Fabien Salsac
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Zsolt Kelemen
Gregory Mouille
Loic Lepiniec

Résumé

Flavonoids are secondary metabolites that fulfil a multitude of functions during the plant life cycle. In Arabidopsis proanthocyanidins (PAs) are flavonoids that specifically accumulate in the innermost integuments of the seed testa (i.e. endothelium), as well as in the chalaza and micropyle areas, and play a vital role in protecting the embryo against various biotic and abiotic stresses. PAs accumulation in the endothelium requires the activity of the MADS box transcription factor TRANSPARENT TESTA (TT) 16 (ARABIDOPSIS B-SISTER/AGAMOUS-LIKE 32) and the UDPglycosyltransferase TT15 (UGT80B1). Interestingly tt16 and tt15 mutants display a very similar flavonoid profiles and patterns of PA accumulation. By using a combination of genetic, molecular, biochemical, and histochemical methods, we showed that both TT16 and TT15 act upstream the PA biosynthetic pathway, but through two distinct genetic routes. We also demonstrated that the activity of TT16 in regulating cell fate determination and PA accumulation in the endothelium is required in the chalaza prior to the globular stage of embryo development. Finally this study provides new insight showing that TT16 and TT15 functions extend beyond PA biosynthesis in the inner integuments of the Arabidopsis seed coat.
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hal-01536007 , version 1 (26-05-2020)

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Wenjia Xu, Sophie Bobet, José Le Gourrierec, Damaris Grain, Délphine de Vos, et al.. TRANSPARENT TESTA 16 and 15 act through different mechanisms to control proanthocyanidin accumulation in Arabidopsis testa. Journal of Experimental Botany, 2017, 68 (11), ⟨10.1093/jxb/erx151⟩. ⟨hal-01536007⟩
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