Global gene expression in muscle from fasted/refed trout reveals up-regulation of genes promoting myofibre hypertrophy but not myofibre production - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue BMC Genomics Année : 2017

Global gene expression in muscle from fasted/refed trout reveals up-regulation of genes promoting myofibre hypertrophy but not myofibre production

Résumé

Background: Compensatory growth is a phase of rapid growth, greater than the growth rate of control animals,that occurs after a period of growth-stunting conditions. Fish show a capacity for compensatory growth afteralleviation of dietary restriction, but the underlying cellular mechanisms are unknown. To learn more about thecontribution of genes regulating hypertrophy (an increase in muscle fibre size) and hyperplasia (the generation ofnew muscle fibres) in the compensatory muscle growth response in fish, we used high-density microarray analysis to investigate the global gene expression in muscle of trout during a fasting-refeeding schedule and in muscle of control-fed trout displaying normal growth.Results: The compensatory muscle growth signature, as defined by genes up-regulated in muscles of refed troutcompared with control-fed trout, showed enrichment in functional categories related to protein biosynthesis andmaturation, such as RNA processing, ribonucleoprotein complex biogenesis, ribosome biogenesis, translation and protein folding. This signature was also enriched in chromatin-remodelling factors of the protein arginine N-methyl transferase family. Unexpectedly, functional categories related to cell division and DNA replication were not inferred from the molecular signature of compensatory muscle growth, and this signature contained virtually none of the genes previously reported to be up-regulated in hyperplastic growth zones of the late trout embryo myotome and to potentially be involved in production of new myofibres, notably genes encoding myogenic regulatory factors, transmembrane receptors essential for myoblast fusion or myofibrillar proteins predominant in nascent myofibres.Conclusion: Genes promoting myofibre growth, but not myofibre formation, were up-regulated in muscles of refedtrout compared with continually fed trout. This suggests that a compensatory muscle growth response, resulting from the stimulation of hypertrophy but not the stimulation of hyperplasia, occurs in trout after refeeding. The generation of a large set of genes up-regulated in muscle of refed trout may yield insights into the molecular and cellular mechanisms controlling skeletal muscle mass in teleost and serve as a useful list of potential molecular markers of muscle growth in fish.
Fichier principal
Vignette du fichier
Rescan_2017_BMC_Genomics_{11DEE2A2-3801-48AA-8232-0D06746228AC}.pdf (912.64 Ko) Télécharger le fichier
Origine : Accord explicite pour ce dépôt
Loading...

Dates et versions

hal-01535201 , version 1 (08-06-2017)

Licence

Paternité - Partage selon les Conditions Initiales

Identifiants

Citer

Pierre-Yves Rescan, Aurélie Le Cam, Cécile Ralliere, Jérôme Montfort. Global gene expression in muscle from fasted/refed trout reveals up-regulation of genes promoting myofibre hypertrophy but not myofibre production. BMC Genomics, 2017, 18 (1), pp.1-12. ⟨10.1186/s12864-017-3837-9⟩. ⟨hal-01535201⟩
46 Consultations
145 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More