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Communication Dans Un Congrès Année : 2016

The power of GWAS to detect natural genetic variation associated with pathogeny in Zymoseptoria tritici

Résumé

Association genetics is a powerful approach to search for the genetic basis of complex traits, including plant diseases caused by pathogens. The goal of our study is to shed light on new genetic components that are associated with natural phenotypic variation in population samples of Zymoseptoria tritici. The traits we are mostly interested in are the disease symptoms caused by Zt on wheat leaves in laboratory conditions. We used a panel of french strains to perform a Genome Wide Association Study. Second generation sequencing data were obtained using Illumina technology, in order to extract SNPs and short indels. Our phenotypic measurements corresponded to: (1) the percent of leaf area covered with pycnidia and the total number of pycnidia at 21 days post-inoculation; (2) the ability of the strains to grow and penetrate the leaf surface between 4 and 5 days post-inoculation, which was obtained using fluorescent microscopy. We used ANOVA models to estimate the adjusted means of our quantitative traits. For the macroscopic traits (leaf area with pycnidia and number of pycnidia), we also compared models which included or not the ability of the strains to develop at the leaf surface post-inoculation, in order to disentangle the host-pathogen interaction between the inner and outer compartments of the plant. GWAS was performed using genome-wide efficient mixed model association. Such a model accounts for the relatedness among individuals. Significant statistical results targeting candidate genes will be presented
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Dates et versions

hal-01531629 , version 1 (01-06-2017)

Identifiants

  • HAL Id : hal-01531629 , version 1
  • PRODINRA : 352330

Citer

Anne Genissel, Marie-Pierre Guillot, Johann J. Confais, Lilian Gout, Marc-Henri Lebrun. The power of GWAS to detect natural genetic variation associated with pathogeny in Zymoseptoria tritici. 9.International symposium on Septoria diseases of cereals, Apr 2016, Paris, France. p.17. ⟨hal-01531629⟩
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