Optical sensing of mechanical pressure based on diffusion measurement in polyacrylamide cell-like barometers
Résumé
Diffusion and transport of small molecules within hydrogel networks are of high interest for i.e. biomedical and pharmaceutical research. In here, using fluorescence correlation spectroscopy (FCS) we experimentally show that the diffusion time in the hydrogel is directly related to the mechanical state
(compression or swelling), and thus to the volume fraction of the gel. Following this observation, we develop cell-like barometers in a form of PAA microbeads, which when incorporated in between cells and combined with a diffusion-based optical readout, can serve as the first biosensors to measure the local pressure inside a growing biological tissues. To illustrate the potential of our method, we use multicellular spheroids (MCS) as a tissue model, and we show that the growth-associated tissue stress is lower than 1 kPa, but increases significantly when an external compressive stress is applied.
Domaines
Biophysique [physics.bio-ph]
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10) Optical sensing of mechanical pressure based on diffusion measurement in polyacrylamide cell-like barometers.pdf (1.22 Mo)
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