Successful chondrogenesis within scaffolds, using magnetic stem cell confinement and bioreactor maturation - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Acta Biomaterialia Année : 2016

Successful chondrogenesis within scaffolds, using magnetic stem cell confinement and bioreactor maturation

Résumé

Tissue engineering strategies, such as cellularized scaffolds approaches, have been explored for cartilage replacement. The challenge, however, remains to produce a cartilaginous tissue incorporating functional chondrocytes and being large and thick enough to be compatible with the replacement of articular defects. Here, we achieved unprecedented cartilage tissue production into a porous polysaccharide scaffold by combining of efficient magnetic condensation of mesenchymal stem cells, and dynamic maturation in a bioreactor. In optimal conditions, all the hallmarks of chondrogenesis were enhanced with a 50-fold increase in collagen II expression compared to negative control, an overexpression of aggrecan and collagen XI, and a very low expression of collagen I and RUNX2. Histological staining showed a large number of cellular aggregates, as well as an increased proteoglycan synthesis by chondrocytes. Interestingly, electron microscopy showed larger chondrocytes and a more abundant extracellular matrix. In addition, the periodicity of the neosynthesized collagen 2 fibers matched that of collagen II. These results represent a major step forward in replacement tissue for cartilage defects.
Fichier principal
Vignette du fichier
Luciani et al.pdf (985.25 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01519129 , version 1 (05-05-2017)

Licence

Paternité

Identifiants

Citer

Nathalie Luciani, Vicard Du, Florence Gazeau, Alain Richert, Didier Letourneur, et al.. Successful chondrogenesis within scaffolds, using magnetic stem cell confinement and bioreactor maturation. Acta Biomaterialia, 2016, 37, pp.101-110. ⟨10.1016/j.actbio.2016.04.009⟩. ⟨hal-01519129⟩
165 Consultations
811 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More