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Article Dans Une Revue Cytometry Part A Année : 2015

Evaluating the Efficiency of Isotope Transmission for Improved Panel Design and a Comparison of the Detection Sensitivities of Mass Cytometer Instruments

Sabine Tricot
  • Fonction : Auteur
Mickael Meyrand
  • Fonction : Auteur
Chiara Sammicheli
  • Fonction : Auteur
Jamila Elhmouzi-Younes
  • Fonction : Auteur
Sylvie Bertholet
  • Fonction : Auteur
Marie Malissen
Sandra Nuti
  • Fonction : Auteur

Résumé

The recent introduction of mass cytometry, a technique coupling a cell introduction system generating a stream of single cells with mass spectrometry, has greatly increased the number of parameters that can be measured per single cell. As with all new technology there is a need for dissemination of standardization and quality control procedures. Here, we characterize variations in sensitivity observed across the mass range of a mass cytometer, using different lanthanide tags. We observed a five-fold difference in lanthanide detection over the mass range and demonstrated that each instrument has its own sensitivity pattern. Therefore, the selection of lanthanide combinations is a key step in the establishment of a staining panel for mass cytometry-based experiments, particularly for multicenter studies. We propose the sensitivity pattern as the basis for panel design, instrument standardization and future implementation of normalization algorithms.

Dates et versions

hal-01515301 , version 1 (27-04-2017)

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Citer

Sabine Tricot, Mickael Meyrand, Chiara Sammicheli, Jamila Elhmouzi-Younes, Aurélien Corneau, et al.. Evaluating the Efficiency of Isotope Transmission for Improved Panel Design and a Comparison of the Detection Sensitivities of Mass Cytometer Instruments. Cytometry Part A, 2015, 87A (4), pp.357-368. ⟨10.1002/cyto.a.22648⟩. ⟨hal-01515301⟩
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