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Article Dans Une Revue Blood Année : 2017

iNKT and Memory B-Cell Alterations in HHV-8 Multicentric Castleman Disease

Résumé

Human herpesvirus 8 (HHV-8) is the causative agent of Kaposi sarcoma (KS) and multicentric Castleman disease (MCD), a life-threatening, virally induced B-cell lymphoproliferative disorder. HHV-8 is a B-lymphotropic γ-herpesvirus closely related to the Epstein-Barr virus (EBV). Invariant natural killer T (iNKT) cells are innate-like T cells that play a role in antiviral immunity, specifically in controlling viral replication in EBV-infected B cells. Decline of iNKT cells is associated with age or HIV infection, both situations associated with HHV-8\textendashrelated diseases. We analyzed iNKT cells in both blood (n = 26) and spleen (n = 9) samples from 32 patients with HHV-8 MCD and compared them with patients with KS (n = 24) and healthy donors (n = 29). We determined that both circulating and splenic iNKT cell frequencies were markedly decreased in patients with HHV-8 MCD and were undetectable in 6 of them. Moreover, iNKT cells from patients with HHV-8 MCD displayed a proliferative defect after stimulation with α-galactosylceramide. These iNKT cell alterations were associated with an imbalance in B-cell subsets, including a significant decrease in memory B cells, particularly of marginal zone (MZ) B cells. Coculture experiments revealed that the decrease in iNKT cells contributed to the alterations in the B-cell subset distribution. These observations contribute to a better understanding of the complex interactions between HHV-8 and immune cells that cause HHV-8\textendashrelated MCD.

Dates et versions

hal-01515291 , version 1 (27-04-2017)

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Citer

Zineb Sbihi, Antoine Dossier, David Boutboul, Lionel Galicier, Christophe Parizot, et al.. iNKT and Memory B-Cell Alterations in HHV-8 Multicentric Castleman Disease. Blood, 2017, 129 (7), pp.855--865. ⟨10.1182/blood-2016-06-719716⟩. ⟨hal-01515291⟩
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