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Article Dans Une Revue Journal of Cachexia, Sarcopenia and Muscle Année : 2016

Docosahexaenoic acid-supplementation prior to fasting prevents muscle atrophy in mice

Résumé

Background : Muscle wasting prevails in numerous diseases (e.g. diabetes, cardiovascular and kidney diseases, COPD,…) and increases healthcare costs. A major clinical issue is to devise new strategies preventing muscle wasting. We hypothesized that 8-week docosahexaenoic acid (DHA) supplementation prior to fasting may preserve muscle mass in vivo. Methods : Six-week-old C57BL/6 mice were fed a DHA-enriched or a control diet for 8 weeks and then fasted for 48 h. Results : Feeding mice a DHA-enriched diet prior to fasting elevated muscle glycogen contents, reduced muscle wasting, blocked the 55% decrease in Akt phosphorylation, and reduced by 30–40% the activation of AMPK, ubiquitination, or autophagy. The DHA-enriched diet fully abolished the fasting induced-messenger RNA (mRNA) over-expression of the endocannabinoid receptor-1. Finally, DHA prevented or modulated the fasting-dependent increase in muscle mRNA levels for Rab18, PLD1, and perilipins, which determine the formation and fate of lipid droplets, in parallel with muscle sparing. Conclusions : These data suggest that 8-week DHA supplementation increased energy stores that can be efficiently mobilized, and thus preserved muscle mass in response to fasting through the regulation of Akt- and AMPK-dependent signalling pathways for reducing proteolysis activation. Whether a nutritional strategy aiming at increasing energy status may shorten recovery periods in clinical settings remains to be tested.
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Dates et versions

hal-01512083 , version 1 (21-04-2017)

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Christiane Deval, Frédéric Capel, Brigitte Laillet, Cécile Polge, Daniel Bechet, et al.. Docosahexaenoic acid-supplementation prior to fasting prevents muscle atrophy in mice. Journal of Cachexia, Sarcopenia and Muscle, 2016, 7 (5), pp.587-603. ⟨10.1002/jcsm.12103⟩. ⟨hal-01512083⟩
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