Single walled carbon nanotube length determination by asymmetrical-flow field-flow fractionation hyphenated to multi-angle laser-light scattering
Résumé
Asymmetrical flow field-flow fractionation (AFlFFF) hyphenated to multi-angle laser-light scattering (MALS) was evaluated in order to determine single walled carbon nanotube (SWCNT) length distribution. Fractionation conditions were investigated by examining mobile phase ionic strength and pH, channel components and cross-flow rate. Ammonium nitrate-based mobile phase with 10-5molL-1 ionic strength and pH 6 allows the highest sample recovery (89±3%) to be obtained and the lowest loss of the longest SWCNT. A cross-flow rate of 0.9mLmin-1 leads to avoid any significant membrane-sample interaction. Length was evaluated from gyration radius measured by MALS by comparing SWCNT to prolate ellipsoid. In order to validate the fractionation and the length determination obtained by AFlFFF-MALS, different SWCNT aliquots were collected after fractionation and measured by dynamic light scattering (DLS). AFlFFF is confirmed to operate in normal mode over 100-2000nm length. MALS length determination after fractionation is found to be accurate with 5% RSD. Additionally, a shape analysis was performed by combining gyration and hydrodynamic radii. © 2010 Elsevier B.V.
Mots clés
Ammonium nitrate
Asymmetrical flow field-flow fractionations
Cross flows
Flow field-flow fractionation
Gyration radii
Hydrodynamic radius
Hyphenated techniques
Length determination
Length distributions
Length measurement
Mobile phase
Multiangle laser-light scattering
Normal modes
Shape analysis
Size fractionation
SWCNT
Ammonium compounds
Carbon nanotubes
Dynamic light scattering
Flow fields
Liquid chromatography
Refraction
Scattering
Single-walled carbon nanotubes (SWCN)
Fractionation
ammonium nitrate
phosmet
single walled nanotube
accuracy
asymmetrical flow field flow fractionation
flow rate
hydrodynamics
ionic strength
light scattering
membrane
multi angle laser light scattering
pH
separation technique
validation process
Algorithms
Field Flow
Hydrogen-Ion Concentration
Lasers
Light
Linear Models
Molecular Conformation
Nanotubes
Carbon
Osmolar Concentration
Radiation