Integration of ring nanoelectrodes into microwells for the bioelectrochemical analysis in sub-picolitre volumes - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Sensors and Actuators B: Chemical Année : 2016

Integration of ring nanoelectrodes into microwells for the bioelectrochemical analysis in sub-picolitre volumes

Fadhila Sekli-Belaidi
Emilie Vanhove
  • Fonction : Auteur
  • PersonId : 1005713
William Tiddi
  • Fonction : Auteur
  • PersonId : 1005714
Matthieu Polverel
  • Fonction : Auteur
  • PersonId : 1005715
Gabriel Lemercier
  • Fonction : Auteur
  • PersonId : 1005716
Jérôme Launay
Pascal Dubreuil
Stéphane Arbault
Pierre Temple-Boyer

Résumé

In this work, we report the fabrication and the electrochemical characterization of recessed disk microelectrodes (DME) and ring nanoelectrodes (RNE) integrated in microwell arrays. Such configuration has all advantages of microelectrodes arrays but is more suitable for electrochemical measurement in sub-picolitre volumes (~ 0.3 pL). The technological process based on the reactive ion etching of a SiO2/Ti/Pt/Ti/SiO2 stack is optimized in order to integrate RNE arrays on transparent glass substrate. Multiphysic simulations and electrochemical characterizations are conducted in order to study and improve the amperometric behaviour of recessed ring nanoelectrodes according to their geometry. A good fit is shown between experimental, theoretical and simulation results, allowing full understanding of the electrochemical detection properties of RNE-based microwell arrays. Then, a "generation – collection mode" chronoamperometric approach is proposed to evaluate experimentally the collection ratio of RNE arrays and compare it with simulation results. Finally, first electrochemical characterizations in sub-picolitre volumes are conducted with anti-oxidant species. All these results demonstrate that recessed ring nanoelectrode arrays are fitted to the detection of bio-electrochemical species at the microscale and, consequently to single mitochondrion or single sub-cellular organelle analysis.
Fichier principal
Vignette du fichier
manuscript_ElecWell_vf.pdf (1.56 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01503126 , version 1 (06-04-2017)

Identifiants

Citer

Fadhila Sekli-Belaidi, Emilie Vanhove, William Tiddi, Matthieu Polverel, Gabriel Lemercier, et al.. Integration of ring nanoelectrodes into microwells for the bioelectrochemical analysis in sub-picolitre volumes. Sensors and Actuators B: Chemical, 2016, 232, pp.345-356. ⟨10.1016/j.snb.2016.03.130⟩. ⟨hal-01503126⟩
193 Consultations
183 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More