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Journal Articles Nature Methods Year : 2017

mScarlet: a bright monomeric red fluorescent protein for cellular imaging.

Abstract

We report the engineering of mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). We developed mScarlet starting with a consensus synthetic template and using improved spectroscopic screening techniques; mScarlet's crystal structure reveals a planar and rigidified chromophore. mScarlet outperforms existing red fluorescent proteins as a fusion tag, and it is especially useful as a Förster resonance energy transfer (FRET) acceptor in ratiometric imaging.
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Dates and versions

hal-01494322 , version 1 (23-03-2017)

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Daphne S Bindels, Lindsay Haarbosch, Laura van Weeren, Marten Postma, Katrin E Wiese, et al.. mScarlet: a bright monomeric red fluorescent protein for cellular imaging.. Nature Methods, 2017, 14 (1), pp.53-56. ⟨10.1038/nmeth.4074⟩. ⟨hal-01494322⟩
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