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Article Dans Une Revue Industrial Crops and Products Année : 2012

Rapid quantitative determination of oleuropein in olive leaves (Olea europaea) using mid-infrared spectroscopy combined with chemometric analyses

Résumé

Oleuropein, the major active compound in olive leaf, is well known for its benefits for human health. Oleu-ropein is classically quantified by HPLC, which is time and chemical consuming, laborious and expensive. The aim of this work was to examine the potential of mid-infrared spectroscopy, as a rapid tool, to predict oleuropein content in olive leaf from five Tunisian cultivars (Chemlali, Chetoui, Meski, Sayali and Zarrazi) and one French cultivar (Bouteillan). The reference data of oleuropein content were obtained by the HPLC method. Hundred five samples were analyzed by HPLC and mid-infrared spectroscopy. Samples were randomly divided in a calibration set (73 samples) and in a validation set (32 samples). The spectral data sets were correlated with reference data of oleuropein content by using partial least squares (PLS) regression algorithm. The results showed that the PLS model gave satisfactory model for quantitative prediction of oleuropein content in olive leaf (relative error of prediction = 8.5%). The correlation coefficient was 0.91 and 0.74 for calibration set and validation set, respectively. It can be concluded that mid-infrared spectroscopy constitutes a promising technique for rapid quantification of oleuropein in olive leaf.

Dates et versions

hal-01493473 , version 1 (21-03-2017)

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Citer

Fathia Aouidi, Nathalie Dupuy, Jacques Artaud, Sevastianos Roussos, Monji Msallem, et al.. Rapid quantitative determination of oleuropein in olive leaves (Olea europaea) using mid-infrared spectroscopy combined with chemometric analyses. Industrial Crops and Products, 2012, 37, pp.292 - 297. ⟨10.1016/j.indcrop.2011.12.024⟩. ⟨hal-01493473⟩
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