Surface structuring of titanium oxide thin films by a UV femtosecond laser beam (266 nm, 100 fs) is presented in this paper. Without using laser scanning, the results show the formation of regular dots and laser induced periodic surface structures (LIPSS) with a period close to that of the beam wavelength. These nanostructures seem to be due to free-surface energy minimization. Furthermore, laser irradiation of large homogeneous surfaces by laser scan (5 × 5 mm²) showed more exotic surface morphologies, ranging from regular dots and low spatial frequency LIPSS to microstructures that are completely controlled by the laser fluence and the number of shots. Typically 2D circular dots of 100 nm diameter with two distinct periods (260 and 130 nm) are achieved under a very low fluence of 15 mJ/cm² after 13000 shots.