The role of plant cell wall encapsulation and porosity in regulating lipolysis during the digestion of almond seeds - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Food and Function Année : 2016

The role of plant cell wall encapsulation and porosity in regulating lipolysis during the digestion of almond seeds

Résumé

Previous studies have provided evidence that the physical encapsulation of intracellular nutrients by cell walls of plant foods (i.e. dietary fibre) plays a predominant role in influencing macronutrient bioaccessibility (release) from plant foods during human digestion. One unexplored aspect of this is the extent to which digestive enzymes can pass through the cell-wall barrier and hydrolyse the intracellular lipid in almond seeds. The purpose of the present study was to assess the role played by cell walls in influencing the bioaccessibility and digestibility of almond lipid using a range of techniques. Digestibility experiments were performed on raw and roasted almond cells as well as isolated almond oil bodies using in vitro gastric and duodenal digestion models. Residual triacylglycerols and lipolysis products were extracted after 1 h of incubation and analysed by thin layer chromatography. The lipolysis kinetics of almond cells and oil bodies were also investigated using the pH-stat technique. Finally, the potential penetration of pancreatic lipase through the cell wall matrix was investigated using confocal microscopy. Differences in the rates and extent of lipolysis were clearly seen between almond cells and oil bodies, and these differences were observed regardless of the lipase(s) used. These results also showed that almond cell walls that are completely intact limit lipid digestibility, due to an encapsulation mechanism that hinders the diffusion of lipase into the intracellular environment and lipolysis products out of the cells.
Fichier principal
Vignette du fichier
Grundy 2016 The role of plant.pdf (4.35 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Licence : CC BY - Paternité

Dates et versions

hal-01473703 , version 1 (12-02-2024)

Licence

Paternité

Identifiants

Citer

Myriam M.-L. Grundy, Frédéric Carrière, Alan.R. Mackie, David A Gray, Peter Butterworth, et al.. The role of plant cell wall encapsulation and porosity in regulating lipolysis during the digestion of almond seeds. Food and Function, 2016, 7, pp.69-78. ⟨10.1039/C5FO00758E⟩. ⟨hal-01473703⟩

Collections

CNRS UNIV-AMU
65 Consultations
1 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More