Brown-like adipose progenitors derived from human induced pluripotent stem cells: Identification of critical pathways governing their adipogenic capacity - Archive ouverte HAL
Article Dans Une Revue Scientific Reports Année : 2016

Brown-like adipose progenitors derived from human induced pluripotent stem cells: Identification of critical pathways governing their adipogenic capacity

Julian Contet
  • Fonction : Auteur
Xi Yao
  • Fonction : Auteur
Kran Suknuntha
  • Fonction : Auteur
Karima Annab
  • Fonction : Auteur
Igor I. Slukvin
  • Fonction : Auteur
Annie Ladoux
Christian Dani

Résumé

Human induced pluripotent stem cells (hiPSCs) show great promise for obesity treatment as they represent an unlimited source of brown/brite adipose progenitors (BAPs). However, hiPSC-BAPs display a low adipogenic capacity compared to adult-BAPs when maintained in a traditional adipogenic cocktail. The reasons of this feature are unknown and hamper their use both in cell-based therapy and basic research. Here we show that treatment with TGF beta pathway inhibitor SB431542 together with ascorbic acid and EGF were required to promote hiPSCs-BAP differentiation at a level similar to adult-BAP differentiation. hiPSC-BAPs expressed the molecular identity of adult-UCP1 expressing cells (PAX3, CIDEA, DIO2) with both brown (ZIC1) and brite (CD137) adipocyte markers. Altogether, these data highlighted the critical role of TGF beta pathway in switching off hiPSC-brown adipogenesis and revealed novel factors to unlock their differentiation. As hiPSC-BAPs display similarities with adult-BAPs, it opens new opportunities to develop alternative strategies to counteract obesity.
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Dates et versions

hal-01469074 , version 1 (28-09-2023)

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Anne-Laure Hafner, Julian Contet, Christophe Ravaud, Xi Yao, Phi Villageois, et al.. Brown-like adipose progenitors derived from human induced pluripotent stem cells: Identification of critical pathways governing their adipogenic capacity. Scientific Reports, 2016, 6, ⟨10.1038/srep32490⟩. ⟨hal-01469074⟩
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