Early detection of metallo-beta-lactamase NDM-1-and OXA-23 ă carbapenemase-producing Acinetobacter baumannii in Libyan hospitals - Archive ouverte HAL
Article Dans Une Revue International Journal of Antimicrobial Agents Année : 2016

Early detection of metallo-beta-lactamase NDM-1-and OXA-23 ă carbapenemase-producing Acinetobacter baumannii in Libyan hospitals

Najla Mathlouthi
  • Fonction : Auteur
Allaaeddin Ali El Salabi
  • Fonction : Auteur
Ă Mariem Ben Jomaa-Jemili
  • Fonction : Auteur
Sofiane Bakour
  • Fonction : Auteur
Charbel Al-Bayssari
Ă Abdulaziz A. Zorgani
  • Fonction : Auteur
Abdulmajeed Kraiema
  • Fonction : Auteur
Omar Elahmer
  • Fonction : Auteur
Ă Liliane Okdah
  • Fonction : Auteur
Chedly Chouchani

Résumé

Acinetobacter baumannii is an opportunistic pathogen causing various ă nosocomial infections. The aim of this study was to characterise the ă molecular support of carbapenem-resistant A. baumannii clinical isolates ă recovered from two Libyan hospitals. Bacterial isolates were identified ă by matrix-assisted laser desorption/ionisation time-of-flight mass ă spectrometry (MALDI-TOF/MS). Antibiotic susceptibility testing was ă performed using disk diffusion and Etest methods, and carbapenem ă resistance determinants were studied by PCR amplification and ă sequencing. Multilocus sequence typing (MLST) was performed for typing ă of the isolates. All 36 imipenem-resistant isolates tested were ă identified as A. baumannii. The bla(OXA-23) gene was detected in 29 ă strains (80.6%). The metallo-beta-lactamase bla(NDM-1) gene was ă detected in eight isolates (22.2%), showing dissemination of ă multidrug-resistant (MDR) A. baumannii in Tripoli Medical Center and ă Burn and Plastic Surgery Hospital in Libya, including one isolate that ă co-expressed the bla(OXA-23) gene. MLST revealed several sequence types ă (STs). Imipenem-resistant A. baumannii ST2 was the predominant clone ă (16/36; 44.4%). This study shows that NDM-1 and OXA-23 contribute to ă antibiotic resistance in Libyan hospitals and represents the first ă incidence of the association of these two carbapenemases in an ă autochthonous MDR A. baumannii isolated from patients in Libya, ă indicating that there is a longstanding infection control problem in ă these hospitals. (C) 2016 Elsevier B.V. and International Society of ă Chemotherapy. All rights reserved.
Fichier non déposé

Dates et versions

hal-01465107 , version 1 (10-02-2017)

Identifiants

Citer

Najla Mathlouthi, Allaaeddin Ali El Salabi, Ă Mariem Ben Jomaa-Jemili, Sofiane Bakour, Charbel Al-Bayssari, et al.. Early detection of metallo-beta-lactamase NDM-1-and OXA-23 ă carbapenemase-producing Acinetobacter baumannii in Libyan hospitals. International Journal of Antimicrobial Agents, 2016, 48 (1), pp.46-50. ⟨10.1016/j.ijantimicag.2016.03.007⟩. ⟨hal-01465107⟩

Collections

CNRS UNIV-AMU
60 Consultations
0 Téléchargements

Altmetric

Partager

More