Revisiting the structure/function relationships of H/ACA(-like) RNAs: A unified model for Euryarchaea and Crenarchaea
Résumé
.A structural and functional classification of H/ACA and H/ACA-like motifs is obtained from the analysis of the H/ACA guide RNAs which have been identified previously in the genomes of Euryarchaea (Pyrococcus) and Crenarchaea (Pyrobaculum). A unified structure/function model is proposed based on the common structural determinants shared by H/ACA and H/ACA-like motifs in both Euryarchaea and Crenarchaea. Using a computational approach, structural and energetic rules for the guide:target RNA-RNA interactions are derived from structural and functional data on the H/ACA RNP particles. H/ACA(-like) motifs found in Pyrococcus are evaluated through the classification and their biological relevance is discussed. Extra-ribosomal targets found in both Pyrococcus and Pyrobaculum might support the hypothesis of a gene regulation mediated by H/ACA(-like) guide RNAs in archaea.
Mots clés
proteins
prediction
archaea
models
molecular
nucleotide motifs
base pairing
euryarchaeota
identification
structure-activity relationship
rna
transfer
small nucleolar
families
rna folding
gene expression regulation
archaeal
uridine
pyrococcus abyssi
complex
pyrobaculum
guide
pseudouridine synthase
noncoding rnas
crystal-structure
pyrococcus-abyssi
pseudouridine
haloferax-volcanii
substrate rna
ribonucleoprotein particle
archaeal genome browser
h/aca soluble-rnas