In vitro reconstitution of a trimeric complex of DivIB, DivIC and FtsL, and their transient co-localization at the division site in Streptococcus pneumoniae. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Molecular Microbiology Année : 2004

In vitro reconstitution of a trimeric complex of DivIB, DivIC and FtsL, and their transient co-localization at the division site in Streptococcus pneumoniae.

Résumé

DivIB, DivIC and FtsL are bacterial proteins essential for cell division, which show interdependencies for their stabilities and localization. We have reconstituted in vitro a trimeric complex consisting of the recombinant extracellular domains of the three proteins from Streptococcus pneumoniae. The extracellular domain of DivIB was found to associate with a heterodimer of those of DivIC and FtsL. The heterodimerization of DivIC and FtsL was artificially constrained by fusion with interacting coiled-coils. Immunofluorescence experiments showed that DivIC is always localized at mid-cell, in contrast to DivIB and FtsL, which are co-localized with DivIC only during septation. Taken together, our data suggest that assembly of the trimeric complex DivIB/DivIC/FtsL is regulated during the cell cycle through controlled formation of the DivIC/FtsL heterodimer.
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Dates et versions

hal-01464086 , version 1 (09-02-2017)

Identifiants

  • HAL Id : hal-01464086 , version 1
  • PUBMED : 15659160

Citer

Marjolaine Noirclerc-Savoye, Audrey Le Gouellec, Cécile Morlot, Otto Dideberg, Thierry Vernet, et al.. In vitro reconstitution of a trimeric complex of DivIB, DivIC and FtsL, and their transient co-localization at the division site in Streptococcus pneumoniae.. Molecular Microbiology, 2004, pp.413-24. ⟨hal-01464086⟩
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