Dense fibrillar collagen matrices to analyse extracellular matrix receptor function - Archive ouverte HAL
Article Dans Une Revue Pathologie Biologie Année : 2012

Dense fibrillar collagen matrices to analyse extracellular matrix receptor function

C. Helary
B. Rodrigues-Sanchez
  • Fonction : Auteur
S. Vigier
  • Fonction : Auteur
Marie-Madeleine Giraud-Guille
  • Fonction : Auteur
  • PersonId : 980646

Résumé

Aim. - The goal of this study was to understand whether dense fibrillar collagen matrices, with a hierarchical structure resembling native collagen matrices, could be useful to study collagen receptor function, in a more physiological context. The receptor analysed here was integrin alpha 11 beta 1, already shown to be involved in cell attachment and migration on collagen-coated plastic, and also in contraction of loose fibrillar collagen hydrogels. Materials and methods. - Collagen matrices prepared here corresponded to dense fibrillar hydrogels concentrated at 5 mg/ml. The behaviour of alpha 11 beta 1 deficient fibroblasts seeded on these concentrated matrices was assessed in terms of adhesion, morphology and migration, then compared to that observed on classical hydrogels at 1 mg/ml, corresponding to loose collagen matrices. Results. - Short-term attachment assays showed disturbed interactions between alpha 11 beta 1 deficient cells and collagen matrices in a concentration-dependent manner. Long-term assays revealed reduced cell spreading of alpha 11(-/-) cells on the dense collagen matrices, associated with a disturbed cytoskeleton network. Moreover, anoikis was observed when alpha 11(-/-) cells were seeded on 5 mg/ml matrices, and not on looser 1 mg/ml matrices. In scratch wound in vitro assays, carried out with cells on 5 mg/ml fibrillar collagen matrices, alpha 11(-/-) cells migrated much better than their wild-type counterparts. In contrast, no significant difference was observed between wild and knock-out cells seeded on plastic. Conclusions. - The present study demonstrates the validity of in vivo-like dense fibrillar collagen matrices to evaluate cell receptor functions more significantly than with 2D cell cultures or loose hydrogels.

Dates et versions

hal-01461434 , version 1 (08-02-2017)

Identifiants

Citer

C. Helary, B. Rodrigues-Sanchez, S. Vigier, Marie-Madeleine Giraud-Guille. Dense fibrillar collagen matrices to analyse extracellular matrix receptor function. Pathologie Biologie, 2012, 60 (1), pp.7-14. ⟨10.1016/j.patbio.2011.10.007⟩. ⟨hal-01461434⟩
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