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Article Dans Une Revue Langmuir Année : 2012

Biochemical Investigation of the Formation of Three-Dimensional Networks from DNA-Grafted Large Silica Particles

Jiangyu Wu
  • Fonction : Auteur
Thibaud Coradin
Carole Aimé
  • Fonction : Auteur
  • PersonId : 741160
  • IdHAL : carole-aime

Résumé

DNA is used to rationally build up networks of silica nanoparticles (SiNPs) based on the molecular recognition properties of complementary sequences. Network self-assembly is controlled from DNA covalently grafted at the surface of chemically modified SiNPs. Two strategies are compared, where grafted DNA sequences are designed in a three-strand system using noncomplementary sequences and an extra DNA linker, or in a two-strand approach for direct hybridization. In this paper, both systems are compared in terms of DNA hybridization stability, network size, and three-dimensional organization using a combination of dynamic light scattering and electron microscopy. The observed differences are discussed in terms of hybridization interactions between DNA sequences in particle-free systems through fluorescence, circular dichroism, and UV spectroscopy techniques.

Domaines

Matériaux
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Dates et versions

hal-01461427 , version 1 (08-02-2017)

Identifiants

Citer

Jiangyu Wu, Jérémie Silvent, Thibaud Coradin, Carole Aimé. Biochemical Investigation of the Formation of Three-Dimensional Networks from DNA-Grafted Large Silica Particles. Langmuir, 2012, 28 (4), pp.2156-2165. ⟨10.1021/la2037372⟩. ⟨hal-01461427⟩
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