Immobilization of a Polyphenol Oxidase Extract from Terfezia leonis Tul. Desert Truffle in Multilayered Silica Films for Dopamine Biosensing - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Silicon Année : 2013

Immobilization of a Polyphenol Oxidase Extract from Terfezia leonis Tul. Desert Truffle in Multilayered Silica Films for Dopamine Biosensing

Thomas Moreau
  • Fonction : Auteur
Christophe Depagne
  • Fonction : Auteur
Thibaud Coradin

Résumé

An optical biosensor based on the immobilization of a crude extract from Terfezia leonis Tul. Desert truffle in silica films was developed for dopamine detection. The polyphenol oxidase activity of the extract was first compared with commercial tyrosinase, showing better stability in slightly acidic conditions and under mild heating conditions. The sol-gel encapsulation process was found favourable for the stability and preservation of the enzymatic conversion of dopamine into dopachrome. The range of linear detection of dopamine was 0.1 mM-1.5 mM with a two minutes response time. These results demonstrate the benefits of associating a cost-effective enzymatically-active extract with water-based robust silica hosts for biosensor design.

Domaines

Matériaux

Dates et versions

hal-01461417 , version 1 (08-02-2017)

Identifiants

Citer

Hicham Gouzi, Thomas Moreau, Christophe Depagne, Thibaud Coradin. Immobilization of a Polyphenol Oxidase Extract from Terfezia leonis Tul. Desert Truffle in Multilayered Silica Films for Dopamine Biosensing. Silicon, 2013, 5 (4), pp.241-246. ⟨10.1007/s12633-013-9165-z⟩. ⟨hal-01461417⟩
83 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More