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Article Dans Une Revue Journal of materials chemistry‎ B Année : 2013

Preparation of aqueous sol-gel silica and titania multi-layered thin films and their evaluation as biomolecular encapsulation hosts

Thomas Moreau
  • Fonction : Auteur
Christophe Depagne
  • Fonction : Auteur
Gad Suissa
  • Fonction : Auteur
Thibaud Coradin

Résumé

Multi-layered silica and titania thin films were prepared via an aqueous sol-gel route. The titania layers could efficiently entrap anti-HRP antibodies and showed increase in stability upon ageing, up to 2 months. Specific activity of the entrapped antibodies could be monitored in a reliable manner over one week. The silica films showed lower performances, as a probable consequence of large pore size. Increase in the titania precursor (TiBALDH) concentration and addition of glycerol allowed the design of tri-enzymatic multi-layered systems for the detection of lactose. The multilayer approach showed higher robustness than the co-immobilization of the enzymes in a single layer. This process is simple, solvent-free and flexible, opening the route to multi-biofunctional materials at the nanoscale.

Domaines

Matériaux
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Dates et versions

hal-01461415 , version 1 (08-02-2017)

Identifiants

Citer

Thomas Moreau, Christophe Depagne, Gad Suissa, Hicham Gouzi, Thibaud Coradin. Preparation of aqueous sol-gel silica and titania multi-layered thin films and their evaluation as biomolecular encapsulation hosts. Journal of materials chemistry‎ B, 2013, 1 (9), pp.1235-1240. ⟨10.1039/c2tb00417h⟩. ⟨hal-01461415⟩
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