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Journal Articles EMBO Reports Year : 2014

Type VI secretion and bacteriophage tail tubes share a common assembly pathway.

Abstract

The Type VI secretion system (T6SS) is a widespread macromolecular structure that delivers protein effectors to both eukaryotic and prokaryotic recipient cells. The current model describes the T6SS as an inverted phage tail composed of a sheath-like structure wrapped around a tube assembled by stacked Hcp hexamers. Although recent progress has been made to understand T6SS sheath assembly and dynamics, there is no evidence that Hcp forms tubes in vivo. Here we show that Hcp interacts with TssB, a component of the T6SS sheath. Using a cysteine substitution approach, we demonstrate that Hcp hexamers assemble tubes in an ordered manner with a head-to-tail stacking that are used as a scaffold for polymerization of the TssB/C sheath-like structure. Finally, we show that VgrG but not TssB/C controls the proper assembly of the Hcp tubular structure. These results highlight the conservation in the assembly mechanisms between the T6SS and the bacteriophage tail tube/sheath.

Dates and versions

hal-01458198 , version 1 (06-02-2017)

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Yannick R Brunet, Jérôme Hénin, Hervé H. Celia, E. Cascales. Type VI secretion and bacteriophage tail tubes share a common assembly pathway.. EMBO Reports, 2014, 15 (3), pp.315--21. ⟨10.1002/embr.201337936⟩. ⟨hal-01458198⟩
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