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Article Dans Une Revue European Journal of Lipid Science and Technology Année : 2007

Membrane phospholipids and sterols in microfiltered milk fat globules

Résumé

Native milk fat globules of various mean diameters, ranging from d43 = 2.3 mm to 8.0 mm, were obtained using microfiltration of raw whole milk. After milk fat globule washing, the milk fat globule membrane (MFGM) was separated by manual churning. After total lipid extraction and separation of polar lipids, their phospholipid (PL) and sterol composition was measured using thin-layer chromatography, methyl ester analyses by gas chromatography, and gas chromatography coupled to mass spectrometry. The main PL species were phosphatidylethanolamine, phosphatidylcholine and sphingomyelin. The respective fatty acid composition of each PL species was measured. Many different minor bioactive sterols were detected in the MFGM, e.g.lanosterol, lathosterol, desmosterol, stigmasterol and b-sitosterol. No significant differences in the PL and sterol profile were found between MFGM extracted from small and large milk fat globule fractions.

Dates et versions

hal-01453997 , version 1 (02-02-2017)

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Citer

Caroline Fauquant, Valérie Briard-Bion, Nadine Leconte, Michel Guichardant, Marie-Caroline Michalski. Membrane phospholipids and sterols in microfiltered milk fat globules. European Journal of Lipid Science and Technology, 2007, 109, pp.1167-1173. ⟨10.1002/ejlt.200700119⟩. ⟨hal-01453997⟩
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