We assessed the extent of temporal variation and autocorrelation in fish habitat use based on an experimental study of individual 0+ juvenile barbel, Barbus barbus, in an artificial flume. Five treated and five control fish were individually subjected to an increase in discharge (intervention) halfway through each experiment and kept at baseline discharge throughout, respectively. Preference curves for velocity were generated for each of 60 trials per experiment and for each combination of treated/control (fish) x before/after-intervention. There were large between- and within-individual differences in velocity preference, both in treated and in control fish. Most barbel explored the entire range of velocities, whereas some individuals used a more limited range. Temporal variation in behavioural responses was assessed by a PCA-based methodology. Autocorrelation (i.e. correlation between sequential trials) was diagnosed in most response profiles, supporting recent findings that individuals may have a 'memory' of their past velocity usage. The relevance of the results for numerical habitat models of fish habitat assessment is discussed, as well as the importance of incorporating temporal variability into fish habitat use models (e.g. PHABSIM), not only as ontogenetic intervals but also as longitudinal data of individual behaviours. A warning is also re-issued about the erroneous belief of 'pseudoreplication' simply arising from repeated measurements in time.