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Article Dans Une Revue Environmental and Molecular Mutagenesis Année : 2008

Evaluation of the genotoxicity of river sediments from industrialized and unaffected areas using a battery of short-term bioassays

Résumé

The present investigation evaluated the capacity of the Salmonella mutagenicity test, the comet assay, and the micronucleus assay to detect and characterize the genotoxic profile of river sediments. Three stations were selected on an urban river (Bouches du Rhône, France) exposed to various sources of industrial and urban pollution (StA, StB, and StC) and one station on its tributary (StD). One station in a nonurban river was included (REF). The concentrations of 16 polycyclic aromatic hydrocarbons (PAHs) were determined by HPLC, and the genotoxicity of the sediments was monitored by the Salmonella mutagenicity test (TA98 + S9, YG1041 ± S9), the comet assay, and the micronucleus assay on CHO cells. Chemical analysis showed that the total PAH concentrations ranged from 23 μg kg−1 dw (REF) to 1285 μg kg−1 dw (StD). All the sediments were mutagenic in the Salmonella mutagenicity test. The mutagenicity was probably induced by the presence of nitroarenes (StA, StB, StC, and StD) and aromatic amines (REF) as deduced from the mutagenicity profiles of strains YG1041 ± S9 and TA98 + S9. The comet assay revealed direct DNA lesions in REF, StA, and StB sediments and metabolization-dependent DNA damage in StC and StD. The micronucleus assay showed an absence of clastogenicity for StA ± S9 and StC-S9, and a significant clastogenicity ± S9 for the three other stations. The genotoxicity ranking determined by the comet assay + S9 matched the ranking of total and carcinogenic PAH concentrations, and this assay was found to be the most sensitive.

Dates et versions

hal-01452391 , version 1 (01-02-2017)

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Assia Aouadene, Xavier Moreau, Carole Di Giorgio, Luc Sarrazin, Laetitia de Jong, et al.. Evaluation of the genotoxicity of river sediments from industrialized and unaffected areas using a battery of short-term bioassays. Environmental and Molecular Mutagenesis, 2008, 49 (4), pp.283-299. ⟨10.1002/em.20384⟩. ⟨hal-01452391⟩
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