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Article Dans Une Revue Methods in Molecular Biology Année : 2017

Characterizing Semaphorin Signaling Using Isolated Neurons in Culture

Résumé

Semaphorin guidance molecules act through different receptor complexes to activate multiple signaling cascades leading to changes in axonal growth cone behavior and morphology. We describe here approaches for studying the effect of individual Semaphorins on isolated forebrain neurons from mouse embryos and dissecting downstream signaling pathways. These approaches include the production of recombinant Semaphorin ligands, the culture of dissociated primary neurons, the manipulation of gene expression by electroporation in primary neurons, and functional assays to assess axon outgrowth and growth cone collapse.
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Dates et versions

hal-01446097 , version 1 (25-01-2017)

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Sophie Chauvet, E. Mire, F. Mann. Characterizing Semaphorin Signaling Using Isolated Neurons in Culture. Methods in Molecular Biology, 2017, 1493, pp.223-235. ⟨10.1007/978-1-4939-6448-2_16⟩. ⟨hal-01446097⟩
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