Reconstruction of the Disassembly Pathway of an Icosahedral Viral Capsid and Shape Determination of Two Successive Intermediates. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Physical Chemistry Letters Année : 2015

Reconstruction of the Disassembly Pathway of an Icosahedral Viral Capsid and Shape Determination of Two Successive Intermediates.

Résumé

Viral capsids derived from an icosahedral plant virus widely used in physical and nanotechnological investigations were fully dissociated into dimers by a rapid change of pH. The process was probed in vitro at high spatiotemporal resolution by time-resolved small-angle X-ray scattering using a high brilliance synchrotron source. A powerful custom-made global fitting algorithm allowed us to reconstruct the most likely pathway parametrized by a set of stoichiometric coefficients and to determine the shape of two successive intermediates by ab initio calculations. None of these two unexpected intermediates was previously identified in self-assembly experiments, which suggests that the disassembly pathway is not a mirror image of the assembly pathway. These findings shed new light on the mechanisms and the reversibility of the assembly/disassembly of natural and synthetic virus-based systems. They also demonstrate that both the structure and dynamics of an increasing number of intermediate species become accessible to experiments.
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Dates et versions

hal-01446050 , version 1 (25-01-2017)

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Didier Law-Hine, Anil K Sahoo, Virginie Bailleux, Mehdi Zeghal, Sylvain Prevost, et al.. Reconstruction of the Disassembly Pathway of an Icosahedral Viral Capsid and Shape Determination of Two Successive Intermediates.. Journal of Physical Chemistry Letters, 2015, 6 (17), pp.3471-6. ⟨10.1021/acs.jpclett.5b01478⟩. ⟨hal-01446050⟩
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