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Article Dans Une Revue Acta crystallographica Section D : Structural biology [1993-...] Année : 2016

Online ion-exchange chromatography for small-angle X-ray scattering

Résumé

Biological small-angle X-ray scattering (BioSAXS) is a powerful technique to determine the solution structure, particle size, shape and surface-to-volume ratio of macromolecules. However, a drawback is that the sample needs to be monodisperse. To ensure this, size-exclusion chromatography (SEC) has been implemented on many BioSAXS beamlines. Here, the integration of ion-exchange chromatography (IEC) using both continuous linear and step gradients on a beamline is described. Background subtraction for continuous gradients by shifting a reference measurement and two different approaches for step gradients, which are based on interpolating between two background measurements, are discussed. The results presented here serve as a proof of principle for online IEC and subsequent data treatment.

Dates et versions

hal-01426899 , version 1 (05-01-2017)

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Stephanie Hutin, Martha Brennich, Benoit Maillot, Adam Round. Online ion-exchange chromatography for small-angle X-ray scattering. Acta crystallographica Section D : Structural biology [1993-..], 2016, 72 (10), pp.1090 - 1099. ⟨10.1107/S2059798316012833⟩. ⟨hal-01426899⟩
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