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Article Dans Une Revue FEBS Journal Année : 2016

Calcium channels in chicken sperm regulate motility and the acrosome reaction

Résumé

Intracellular cytoplasmic calcium ([Ca(2+) ]i ) has an important regulatory role in gamete functions. The involved biochemical actors, however, are still unknown in birds, an animal class that has lost functional sperm-specific CatSper channels. In this report, we provide evidence for the presence and expression of different Ca(2+) channels in chicken sperm, including high voltage-activated channels (L and R-type), the Store Operated Ca(2+) channel (SOC) complex component Orai1, transient receptor potential channel (TRPC1) and inositol 1,4,5-trisphosphate receptors (IP3 R1). L and R-types channels were mainly localized in the acrosome and the midpiece, whereas T-type channels were not detected in chicken sperm. Orai1 was found in all compartments but with a weak, diffuse signal in the flagellum. TRCP1 was mainly localized in the acrosome and the midpiece, but was also identified with a weak, diffuse signal in the nucleus and the flagellum. IP3 R1 was mainly detected in the nucleus. The Ca(2+) channel inhibitors Nifedipine (L-type), SNX-482 (R-type), MRS-1845, 2-APB and YM-58483 (SOCs) decreased [Ca(2+) ]i sperm motility and acrosome reaction (AR) capability, with the SOC inhibitors most efficiently inhibiting these functions. Furthermore, we showed that Ca(2+) -mediated induction of AMPK phosphorylation was blocked by SOC inhibition. Our identification of important regulators of Ca(2+) signaling in bird sperm suggests that SOCs have a predominant role in gamete function, whereas T-type channels might not be involved. In addition, Ca(2+) entry via SOCs appears to be the most likely pathway for AMPK activation and consequent energy-requiring sperm functions such as motility and the AR.

Domaines

Autre [q-bio.OT]

Dates et versions

hal-01409315 , version 1 (05-12-2016)

Identifiants

Citer

Thi-Mong-Diep Nguyen, Anne Duittoz, Christophe Praud, Yves Combarnous, Elisabeth Blesbois. Calcium channels in chicken sperm regulate motility and the acrosome reaction. FEBS Journal, 2016, 283 (10), pp.1902-1920. ⟨10.1111/febs.13710⟩. ⟨hal-01409315⟩
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