Deciphering the molecular mechanisms underpinning the transcriptional control of gene expression by L-AFL proteins in Arabidopsis seed. - Archive ouverte HAL
Article Dans Une Revue Plant Physiology Année : 2016

Deciphering the molecular mechanisms underpinning the transcriptional control of gene expression by L-AFL proteins in Arabidopsis seed.

Sebastien S. Baud
Zsolt Kelemen
Johanne Thévenin
Céline Boulard
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Alexandra To
Nathalie Berger
Loic Lepiniec

Résumé

In Arabidopsis (Arabidopsis thaliana), transcriptional control of seed maturation involves three related regulators with a B3domain, namely LEAFY COTYLEDON2 (LEC2), ABSCISIC ACID INSENSITIVE3 (ABI3), and FUSCA3 (ABI3/FUS3/LEC2[AFLs]). Although genetic analyses have demonstrated partially overlapping functions of these regulators, the underlyingmolecular mechanisms remained elusive. The results presented here confirmed that the three proteins bind RY DNA elements(with a 59-CATG-39 core sequence) but with different specificities for flanking nucleotides. In planta as in the moss Physcomitrellapatens protoplasts, the presence of RY-like (RYL) elements is necessary but not sufficient for the regulation of the OLEOSIN1 (OLE1)promoter by the B3AFLs. G box-like domains, located in the vicinity of the RYL elements, also are required for proper activation ofthe promoter, suggesting that several proteins are involved. Consistentwith this idea, LEC2 andABI3 showed synergistic effects onthe activation of the OLE1 promoter. What is more, LEC1 (a homolog of the NF-YB subunit of the CCAAT-binding complex)further enhanced the activation of this target promoter in the presence of LEC2 and ABI3. Finally, recombinant LEC1 and LEC2proteins produced in Arabidopsis protoplasts could form a ternary complex with NF-YC2 in vitro, providing a molecular explanationfor their functional interactions. Taken together, these results allow us to propose a molecularmodel for the transcriptional regulationof seed genes by the L-AFL proteins, based on the formation of regulatory multiprotein complexes between NF-YBs, which carry aspecific aspartate-55 residue, and B3 transcription factors.
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Dates et versions

hal-01406124 , version 1 (02-09-2024)

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Sebastien S. Baud, Zsolt Kelemen, Johanne Thévenin, Céline Boulard, Sandrine Blanchet, et al.. Deciphering the molecular mechanisms underpinning the transcriptional control of gene expression by L-AFL proteins in Arabidopsis seed.. Plant Physiology, 2016, 171 (171), pp.1099-1112. ⟨10.1104/pp.16.00034⟩. ⟨hal-01406124⟩
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