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Article Dans Une Revue Carbohydrate Polymers Année : 2016

Polyelectrolyte complexes via desalting mixtures of hyaluronic acid and chitosan-Physicochemical study and structural analysis

Guillaume Sudre
John Meadows
  • Fonction : Auteur
Sébastien Malaise
  • Fonction : Auteur
Agnès Crépet
Laurent David
Thierry Delair

Résumé

Polyelectrolyte complexes (PECs) were prepared from Chitosan (CS) and Hyaluronic Acid (HYA) homogeneous mixtures of aqueous solutions. The method consisted of preparing a homogeneous mixture of the two polysaccharides via charge screening at high salt concentrations. Then, the mixture was dialyzed, leading to the controlled self -assembly of the two polyelectrolytes. Critical parameters like the chitosan degree of acetylation (DA) and molar mass (Mw), the residual salt concentration and the molar charge ratio r = n(NH3)(+) (CS)In-coo(-) (HYA) accounted for the transition from homogeneous aqueous solutions to colloidal suspensions (r = 0.1) or gel coacervates (r=0.5). The influence of the DA and Mw of CS was evaluated by visual observations, light scattering and rheological measurements. For low values of r, Small lAngle X-ray Scattering (SAXS) experiments revealed that the HYA nanostructure was weakly affected by the presence of PECs. On the contrary, the structure was impacted when increasing r, revealing a heterogeneous aggregate morphology with ladder-like chain interactions.
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Dates et versions

hal-01399369 , version 1 (18-11-2016)

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Citer

Gautier Lalevée, Guillaume Sudre, Alexandra Montembault, John Meadows, Sébastien Malaise, et al.. Polyelectrolyte complexes via desalting mixtures of hyaluronic acid and chitosan-Physicochemical study and structural analysis. Carbohydrate Polymers, 2016, 154, pp.86-95. ⟨10.1016/j.carbpol.2016.08.007⟩. ⟨hal-01399369⟩
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