A fast and robust UHPLC-MRM-MS method to characterize and quantify grape skin tannins after chemical depolymerization
Résumé
A rapid, sensitive, and selective analysis method using ultra high performance liquid
chromatography coupled with triple-quadrupole mass spectrometry (UHPLC-QqQ-MS) has been
developed for the characterization and quantification of grape skin flavan-3-ols after acid-catalysed
depolymerization in the presence of phloroglucinol (phloroglucinolysis). The compound detection
being based on specific MS transitions in Multiple Reaction Monitoring (MRM) mode, this fast
gradient robust method allows analysis of constitutive units of grape skin proanthocyanidins,
including some present in trace amounts, in a single injection, with a throughput of 6 samples
per hour. This method was applied to a set of 214 grape skin samples from 107 different red and
white grape cultivars grown under two conditions in the vineyard, irrigated or non-irrigated. The
results of triplicate analyses confirmed the robustness of the method, which was thus proven to be
suitable for high-throughput and large-scale metabolomics studies. Moreover, these preliminary
results suggest that analysis of tannin composition is relevant to investigate the genetic bases of grape
response to drought.
Origine : Publication financée par une institution
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