Inference of Longevity-Related Genes from a Robust Coexpression Network of Seed Maturation Identifies Regulators Linking Seed Storability to Biotic Defense-Related Pathways - Archive ouverte HAL Access content directly
Journal Articles The Plant cell Year : 2015

Inference of Longevity-Related Genes from a Robust Coexpression Network of Seed Maturation Identifies Regulators Linking Seed Storability to Biotic Defense-Related Pathways

Karima Righetti
  • Function : Author
Joseph Vu
  • Function : Author
Sandra Pelletier
Benoit Ly Vu
  • Function : Author
Enrico Glaab
David Lalanne
  • Function : Author
Asher Pasha
  • Function : Author
Rohan Patel
  • Function : Author
Nicholas Provart
  • Function : Author
Jérôme Verdier
Olivier Leprince

Abstract

Seed longevity, the maintenance of viability during storage, is a crucial factor for preservation of genetic resources and ensuring proper seedling establishment and high crop yield. We used a systems biology approach to identify key genes regulating the acquisition of longevity during seed maturation of Medicago truncatula. Using 104 transcriptomes from seed developmental time courses obtained in five growth environments, we generated a robust, stable coexpression network (MatNet), thereby capturing the conserved backbone of maturation. Using a trait-based gene significance measure, a coexpression module related to the acquisition of longevity was inferred from MatNet. Comparative analysis of the maturation processes in M. truncatula and Arabidopsis thaliana seeds and mining Arabidopsis interaction databases revealed conserved connectivity for 87% of longevity module nodes between both species. Arabidopsis mutant screening for longevity and maturation phenotypes demonstrated high predictive power of the longevity cross-species network. Overrepresentation analysis of the network nodes indicated biological functions related to defense, light, and auxin. Characterization of defense-related wrky3 and nf-x1-like1 (nfxl1) transcription factor mutants demonstrated that these genes regulate some of the network nodes and exhibit impaired acquisition of longevity during maturation. These data suggest that seed longevity evolved by co-opting existing genetic pathways regulating the activation of defense against pathogens.

Dates and versions

hal-01392707 , version 1 (04-11-2016)

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Karima Righetti, Joseph Vu, Sandra Pelletier, Benoit Ly Vu, Enrico Glaab, et al.. Inference of Longevity-Related Genes from a Robust Coexpression Network of Seed Maturation Identifies Regulators Linking Seed Storability to Biotic Defense-Related Pathways. The Plant cell, 2015, 27 (10), pp.2692-2708. ⟨10.1105/tpc.15.00632⟩. ⟨hal-01392707⟩
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