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Article Dans Une Revue Mitochondrion Année : 2015

In vivo time-lapse imaging of mitochondria in healthy and diseased peripheral myelin sheath

Sergio Gonzalez
  • Fonction : Auteur
Ruani Fernando
  • Fonction : Auteur
Jade Berthelot
  • Fonction : Auteur
Claire Perrin-Tricaud
  • Fonction : Auteur
Emmanuelle Sarzi
  • Fonction : Auteur
Roman Chrast
  • Fonction : Auteur
Nicolas Tricaud
  • Fonction : Auteur

Résumé

The myelin sheath that covers a large amount of neurons is critical for their homeostasis, and myelinating glia mitochondria have recently been shown to be essential for neuron survival. However morphological and physiological properties of these organelles remain elusive. Here we report a method to analyze mitochondrial dynamics and morphology in myelinating Schwann cells of living mice using viral transduction and time-lapse multiphoton microscopy. We describe the distribution, shape, size and dynamics of mitochondria in live cells. We also report mitochondrial alterations in Opa1(delTTAG) mutant mice cells at presymptomatic stages, suggesting that mitochondrial defects in myelin contribute to OPA1 related neuropathy and represent a biomarker for the disease.

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Dates et versions

hal-01392227 , version 1 (04-11-2016)

Identifiants

Citer

Sergio Gonzalez, Ruani Fernando, Jade Berthelot, Claire Perrin-Tricaud, Emmanuelle Sarzi, et al.. In vivo time-lapse imaging of mitochondria in healthy and diseased peripheral myelin sheath. Mitochondrion, 2015, 23, pp.32-41. ⟨10.1016/j.mito.2015.05.004⟩. ⟨hal-01392227⟩

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