In vivo time-lapse imaging of mitochondria in healthy and diseased peripheral myelin sheath - Archive ouverte HAL Access content directly
Journal Articles Mitochondrion Year : 2015

In vivo time-lapse imaging of mitochondria in healthy and diseased peripheral myelin sheath

Sergio Gonzalez
  • Function : Author
Ruani Fernando
  • Function : Author
Jade Berthelot
  • Function : Author
Claire Perrin-Tricaud
  • Function : Author
Emmanuelle Sarzi
  • Function : Author
Roman Chrast
  • Function : Author
Nicolas Tricaud
  • Function : Author

Abstract

The myelin sheath that covers a large amount of neurons is critical for their homeostasis, and myelinating glia mitochondria have recently been shown to be essential for neuron survival. However morphological and physiological properties of these organelles remain elusive. Here we report a method to analyze mitochondrial dynamics and morphology in myelinating Schwann cells of living mice using viral transduction and time-lapse multiphoton microscopy. We describe the distribution, shape, size and dynamics of mitochondria in live cells. We also report mitochondrial alterations in Opa1(delTTAG) mutant mice cells at presymptomatic stages, suggesting that mitochondrial defects in myelin contribute to OPA1 related neuropathy and represent a biomarker for the disease.

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Dates and versions

hal-01392227 , version 1 (04-11-2016)

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Sergio Gonzalez, Ruani Fernando, Jade Berthelot, Claire Perrin-Tricaud, Emmanuelle Sarzi, et al.. In vivo time-lapse imaging of mitochondria in healthy and diseased peripheral myelin sheath. Mitochondrion, 2015, 23, pp.32-41. ⟨10.1016/j.mito.2015.05.004⟩. ⟨hal-01392227⟩

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