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Article Dans Une Revue Methods in Molecular Biology Année : 2016

The Physcomitrella patens System for Transient Gene Expression Assays.

Johanne Thévenin
Wenjia Xu
  • Fonction : Auteur
  • PersonId : 1136639
Louise Vaisman
  • Fonction : Auteur
  • PersonId : 991964
Loic Lepiniec
Bertrand Dubreucq

Résumé

Transient expression assays are valuable techniques to study in vivo the transcriptional regulation of gene expression. These methods allow to assess the transcriptional properties of a given transcription factor (TF) or a complex of regulatory proteins against specific DNA motifs, called cis-regulatory elements. Here, we describe a fast, efficient, and reliable method based on the use of Physcomitrella patens protoplasts that allows the study of gene expression in a qualitative and quantitative manner by combining the advantage of GFP (green fluorescent protein) as a marker of promoter activity with flow cytometry for accurate measurement of fluorescence in individual cells.
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Dates et versions

hal-01389059 , version 1 (27-10-2016)

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Johanne Thévenin, Wenjia Xu, Louise Vaisman, Loic Lepiniec, Bertrand Dubreucq, et al.. The Physcomitrella patens System for Transient Gene Expression Assays.. Methods in Molecular Biology, 2016, 1482, pp.151-61. ⟨10.1007/978-1-4939-6396-6_10⟩. ⟨hal-01389059⟩
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