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Article Dans Une Revue Metabolomics Année : 2014

Development of an NMR metabolomics-based tool for selection of flaxseed varieties

Résumé

Flaxseed is an important source of lignans and ω-3 fatty acids, compounds which present interest in human health with many applications in food industry. It is therefore necessary to precisely know the metabolite content in flaxseed. A metabolomic approach using NMR was developed to achieve this goal. Due to particular characteristics of flaxseed (high level in oil, high amount in mucilage, and integration of the phenolics into a macromolecule), the extraction procedure had first to be optimized using an experimental design, based on the extraction time, in a water bath or an ultrasound bath, alkaline treatment, defatting, and centrifugation temperature. This methodology was then applied to several flaxseed varieties classified in function of their content in ω-3 fatty acid. The main differences in semi-polar metabolites between these varieties concern compounds of the phenylpropanoid pathway. Hydroxycinnamic acid glucoside and lignan content increase when ω-3 fatty acid content decrease whereas flavonoid content increase in the same way of ω-3 fatty acids.
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Dates et versions

hal-01344388 , version 1 (11-07-2016)

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A. Ramsay, O. Fliniaux, J. Fang, R. Molinie, A. Roscher, et al.. Development of an NMR metabolomics-based tool for selection of flaxseed varieties. Metabolomics, 2014, 10, pp.1258-1267. ⟨10.1007/s11306-014-0664-8⟩. ⟨hal-01344388⟩
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