Genome-wide association study and biological pathway analysis of the Eimeria maxima response in broilers - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Genetics Selection Evolution Année : 2015

Genome-wide association study and biological pathway analysis of the Eimeria maxima response in broilers

Edin Hamzić
  • Fonction : Auteur
Bart Buitenhuis
  • Fonction : Auteur
  • PersonId : 984746
Frédéric Hérault
Rachel Hawken
  • Fonction : Auteur
Mitchel S. Abrahamsen
  • Fonction : Auteur
  • PersonId : 984748
Bertrand Servin
Jean-Michel Elsen
  • Fonction : Auteur
Marie-Hélène Pinard-van Der Laan
Bertrand Bed’hom
Connectez-vous pour contacter l'auteur

Résumé

Background Coccidiosis is the most common and costly disease in the poultry industry and is caused by protozoans of the Eimeria genus. The current control of coccidiosis, based on the use of anticoccidial drugs and vaccination, faces serious obstacles such as drug resistance and the high costs for the development of efficient vaccines, respectively. Therefore, the current control programs must be expanded with complementary approaches such as the use of genetics to improve the host response to Eimeria infections. Recently, we have performed a large-scale challenge study on Cobb500 broilers using E. maxima for which we investigated variability among animals in response to the challenge. As a follow-up to this challenge study, we performed a genome-wide association study (GWAS) to identify genomic regions underlying variability of the measured traits in the response to Eimeria maxima in broilers. Furthermore, we conducted a post-GWAS functional analysis to increase our biological understanding of the underlying response to Eimeria maxima challenge.ResultsIn total, we identified 22 single nucleotide polymorphisms (SNPs) with q value <0.1 distributed across five chromosomes. The highly significant SNPs were associated with body weight gain (three SNPs on GGA5, one SNP on GGA1 and one SNP on GGA3), plasma coloration measured as optical density at wavelengths in the range 465–510 nm (10 SNPs and all on GGA10) and the percentage of β2-globulin in blood plasma (15 SNPs on GGA1 and one SNP on GGA2). Biological pathways related to metabolic processes, cell proliferation, and primary innate immune processes were among the most frequent significantly enriched biological pathways. Furthermore, the network-based analysis produced two networks of high confidence, with one centered on large tumor suppressor kinase 1 (LATS1) and 2 (LATS2) and the second involving the myosin heavy chain 6 (MYH6).ConclusionsWe identified several strong candidate genes and genomic regions associated with traits measured in response to Eimeria maxima in broilers. Furthermore, the post-GWAS functional analysis indicates that biological pathways and networks involved in tissue proliferation and repair along with the primary innate immune response may play the most important role during the early stage of Eimeria maxima infection in broilers.

Mots clés

Fichier principal
Vignette du fichier
12711_2015_Article_170.pdf (1.87 Mo) Télécharger le fichier
12711_2015_170_MOESM10_ESM.xlsx (26.26 Ko) Télécharger le fichier
12711_2015_170_MOESM11_ESM.pdf (2.26 Mo) Télécharger le fichier
12711_2015_170_MOESM12_ESM.pdf (14.05 Ko) Télécharger le fichier
12711_2015_170_MOESM1_ESM.xlsx (8.56 Ko) Télécharger le fichier
12711_2015_170_MOESM2_ESM.xlsx (9.6 Ko) Télécharger le fichier
12711_2015_170_MOESM3_ESM.xlsx (42.05 Mo) Télécharger le fichier
12711_2015_170_MOESM4_ESM.xlsx (283.37 Ko) Télécharger le fichier
12711_2015_170_MOESM5_ESM.xlsx (16.27 Ko) Télécharger le fichier
12711_2015_170_MOESM6_ESM.xlsx (14.78 Ko) Télécharger le fichier
12711_2015_170_MOESM7_ESM.pdf (200.75 Ko) Télécharger le fichier
12711_2015_170_MOESM8_ESM.pdf (202.44 Ko) Télécharger le fichier
12711_2015_170_MOESM9_ESM.pdf (198.09 Ko) Télécharger le fichier
Origine : Publication financée par une institution
Origine : Publication financée par une institution
Origine : Publication financée par une institution
Origine : Publication financée par une institution
Origine : Publication financée par une institution
Origine : Publication financée par une institution
Origine : Publication financée par une institution
Origine : Publication financée par une institution
Origine : Publication financée par une institution
Origine : Publication financée par une institution
Origine : Publication financée par une institution
Origine : Publication financée par une institution
Origine : Publication financée par une institution

Dates et versions

hal-01341325 , version 1 (04-07-2016)

Licence

Paternité

Identifiants

Citer

Edin Hamzić, Bart Buitenhuis, Frédéric Hérault, Rachel Hawken, Mitchel S. Abrahamsen, et al.. Genome-wide association study and biological pathway analysis of the Eimeria maxima response in broilers. Genetics Selection Evolution, 2015, 47 (1), pp.91. ⟨10.1186/s12711-015-0170-0⟩. ⟨hal-01341325⟩
17 Consultations
93 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More