In a context of a changing world, we need to adapt our cultivated plants for food production to the new environmental constraints in order to achieve a more sustainable agriculture. For this purpose, genetic diversity can be used to introduce new original alleles for genes of agronomical interest in elite lines through introgression using recombination as tool for genetic shuffling.However, in bread wheat like in many species with large genome, recombination occurs almost exclusively in distal telomeric regions. Thus, many genes contained in pericentromeric regions which represent 80% of the chromosomes are not admixed during meiosis. At the present time, chromosome 3B of bread wheat is the first wheat chromosome sequenced and organized into an anchored and annotated pseudo-molecule which represents roughly 800Mb. This pseudo-molecule could allow us to investigate relationships between sequence features and recombination hot spots and linkage disequilibrium. To understand the mechanisms that drive recombination at sequence level we precisely mapped recombination hot spots using 1271 Chinese Spring X Renan (CsRe) progeny (F6-SSD). Then we used two collections of 90 lines corresponding to Asian and European genetic pools including Chinese Spring (Asian) and Renan (European) to study the variation of linkage disequilibrium at fine scale in the regions covering our proviseure detected hot spots. At the present time, 30 recombination hot spots were detected in regions of less than 10kb. These hot spots will be compared to sequence features (Epigenetic landmarks, genes, transposable elements, specific motifs) available for chromosome 3B. Concerning linkage disequilibrium, our first results showed a rough decrease of LD in our two populations in a region bearing a hot spot with some differences of linkage between surrounding markers.