<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-01307119</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-19T08:03:01+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Backbone resonance assignments of the m1A22 tRNA methyltransferase TrmK from Bacillus subtilis</title>
            <author role="aut">
              <persName>
                <forename type="first">Clément</forename>
                <surname>Dégut</surname>
              </persName>
              <idno type="halauthorid">1022584-0</idno>
              <affiliation ref="#struct-1184"/>
            </author>
            <author role="crp">
              <persName>
                <forename type="first">Pierre</forename>
                <surname>Barraud</surname>
              </persName>
              <email type="md5">9837e25c4537960388db247c899ec086</email>
              <email type="domain">mol.biol.ethz.ch</email>
              <idno type="idhal" notation="string">pierre-barraud</idno>
              <idno type="idhal" notation="numeric">759321</idno>
              <idno type="halauthorid" notation="string">267161-759321</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-4460-8360</idno>
              <affiliation ref="#struct-1184"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Valéry</forename>
                <surname>Larue</surname>
              </persName>
              <email type="md5">63cf5dff7399c911e94cd5864d1f321f</email>
              <email type="domain">parisdescartes.fr</email>
              <idno type="idhal" notation="string">valery-larue</idno>
              <idno type="idhal" notation="numeric">179987</idno>
              <idno type="halauthorid" notation="string">47021-179987</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-5104-9969</idno>
              <affiliation ref="#struct-1184"/>
            </author>
            <author role="crp">
              <persName>
                <forename type="first">Carine</forename>
                <surname>Tisné</surname>
              </persName>
              <email type="md5">03d9a806f4e3787b4a822e335f929d91</email>
              <email type="domain">cnrs.fr</email>
              <idno type="idhal" notation="string">carine-tisne</idno>
              <idno type="idhal" notation="numeric">981477</idno>
              <idno type="halauthorid" notation="string">135708-981477</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-5534-4650</idno>
              <idno type="IDREF">https://www.idref.fr/131351834</idno>
              <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=kNgdnLkAAAAJ</idno>
              <affiliation ref="#struct-1184"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Pierre</forename>
                <surname>Barraud</surname>
              </persName>
              <email type="md5">bd0b9eefa2123f4c9a54ce1482a81351</email>
              <email type="domain">cnrs.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2016-04-26 16:11:12</date>
              <date type="whenWritten">2016-02-17</date>
              <date type="whenModified">2025-02-26 14:17:44</date>
              <date type="whenReleased">2016-04-26 16:13:12</date>
              <date type="whenProduced">2016-04-20</date>
              <date type="whenEndEmbargoed">2016-04-26</date>
              <ref type="file" target="https://hal.science/hal-01307119v1/document">
                <date notBefore="2016-04-26"/>
              </ref>
              <ref type="file" subtype="author" n="1" target="https://hal.science/hal-01307119v1/file/Degut_BiomolNMRAssign_2016.pdf" id="file-1307119-1387639">
                <date notBefore="2016-04-26"/>
              </ref>
              <ref type="externalLink" target="https://hal.archives-ouvertes.fr/hal-01307119/document"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="138117">
                <persName>
                  <forename>Pierre</forename>
                  <surname>Barraud</surname>
                </persName>
                <email type="md5">bd0b9eefa2123f4c9a54ce1482a81351</email>
                <email type="domain">cnrs.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-01307119</idno>
            <idno type="halUri">https://hal.science/hal-01307119</idno>
            <idno type="halBibtex">degut:hal-01307119</idno>
            <idno type="halRefHtml">&lt;i&gt;Biomolecular NMR Assignments&lt;/i&gt;, 2016, &lt;a target="_blank" href="https://dx.doi.org/10.1007/s12104-016-9680-0"&gt;&amp;#x27E8;10.1007/s12104-016-9680-0&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Biomolecular NMR Assignments, 2016, &amp;#x27E8;10.1007/s12104-016-9680-0&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://about.hal.science/hal-authorisation-v1/">HAL Authorization<ref corresp="#file-1307119-1387639"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="UNIV-PARIS5" corresp="UNIV-PARIS">Université Paris Descartes (Paris 5)</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="INC-CNRS">Institut de Chimie du CNRS</idno>
            <idno type="stamp" n="USPC">Université Sorbonne Paris Cité</idno>
            <idno type="stamp" n="UNIV-PARIS">Université Paris Cité</idno>
            <idno type="stamp" n="TEST2-HALCNRS">TEST2-HALCNRS</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Backbone resonance assignments of the m1A22 tRNA methyltransferase TrmK from Bacillus subtilis</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Clément</forename>
                    <surname>Dégut</surname>
                  </persName>
                  <idno type="halauthorid">1022584-0</idno>
                  <affiliation ref="#struct-1184"/>
                </author>
                <author role="crp">
                  <persName>
                    <forename type="first">Pierre</forename>
                    <surname>Barraud</surname>
                  </persName>
                  <email type="md5">9837e25c4537960388db247c899ec086</email>
                  <email type="domain">mol.biol.ethz.ch</email>
                  <idno type="idhal" notation="string">pierre-barraud</idno>
                  <idno type="idhal" notation="numeric">759321</idno>
                  <idno type="halauthorid" notation="string">267161-759321</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-4460-8360</idno>
                  <affiliation ref="#struct-1184"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Valéry</forename>
                    <surname>Larue</surname>
                  </persName>
                  <email type="md5">63cf5dff7399c911e94cd5864d1f321f</email>
                  <email type="domain">parisdescartes.fr</email>
                  <idno type="idhal" notation="string">valery-larue</idno>
                  <idno type="idhal" notation="numeric">179987</idno>
                  <idno type="halauthorid" notation="string">47021-179987</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-5104-9969</idno>
                  <affiliation ref="#struct-1184"/>
                </author>
                <author role="crp">
                  <persName>
                    <forename type="first">Carine</forename>
                    <surname>Tisné</surname>
                  </persName>
                  <email type="md5">03d9a806f4e3787b4a822e335f929d91</email>
                  <email type="domain">cnrs.fr</email>
                  <idno type="idhal" notation="string">carine-tisne</idno>
                  <idno type="idhal" notation="numeric">981477</idno>
                  <idno type="halauthorid" notation="string">135708-981477</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-5534-4650</idno>
                  <idno type="IDREF">https://www.idref.fr/131351834</idno>
                  <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=kNgdnLkAAAAJ</idno>
                  <affiliation ref="#struct-1184"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">53896</idno>
                <idno type="issn">1874-2718</idno>
                <idno type="eissn">1874-270X</idno>
                <title level="j">Biomolecular NMR Assignments</title>
                <imprint>
                  <publisher>Springer</publisher>
                  <date type="datePub">2016-04-20</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1007/s12104-016-9680-0</idno>
              <idno type="pubmed">27098549</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en"> m1A</term>
                <term xml:lang="en"> tRNA</term>
                <term xml:lang="en"> Unlabeling</term>
                <term xml:lang="en"> TrmK</term>
                <term xml:lang="en"> Methyltransferase</term>
                <term xml:lang="en"> Heteronuclear NMR</term>
                <term xml:lang="en">Backbone resonance assignment</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv.bbm.bs">Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM]</classCode>
              <classCode scheme="halDomain" n="sdv.bbm.bc">Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>RNA modification is a post-transcriptional process by which certain nucleotides are altered after their initial incorporation into an RNA chain. Transfer RNAs (tRNAs) is the most heavily modified class of RNA molecules. These modifications expand the chemical and functional diversity of tRNAs and enhance their structural stability. To date, more than 100 modifications have been identified, the majority of which are specific from one domain of life. However, few modifications are extensively present in the three domains of life. Among those, the m1A nucleotide, which consists in the methylation at position 1 of the adenine aromatic ring, is found in tRNAs and ribosomal RNAs. In tRNAs, the m1A modification occurs at position 9, 14, 22, 57 and 58. The enzyme TrmK catalyzes the m1A formation at position 22. Here we report the backbone 1H, 15N and 13C chemical shift assignments of TrmK from Bacillus subtilis obtained by heteronuclear multidimensional NMR spectroscopy as well as its secondary structure in solution as predicted by TALOS+. These assignments of TrmK pave the way for interaction studies with its tRNA substrates.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-1184" status="OLD">
          <idno type="ISNI">0000 0004 0370 3721</idno>
          <idno type="RNSR">200012689X</idno>
          <idno type="ROR">https://ror.org/0098sx518</idno>
          <orgName>Laboratoire de cristallographie et RMN biologiques</orgName>
          <orgName type="acronym">LCRB - UMR 8015</orgName>
          <date type="start">2000-01-01</date>
          <date type="end">2018-12-31</date>
          <desc>
            <address>
              <addrLine>Faculté des Sciences pharmacologiques et Biologiques - 4, av. de l’Observatoire 75270 PARIS CEDEX 06</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://lcrbw.pharmacie.univ-paris5.fr/</ref>
          </desc>
          <listRelation>
            <relation name="UMR 8015" active="#struct-301664" type="direct"/>
            <relation active="#struct-341038" type="direct"/>
            <relation name="UMR 8015" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-301664" status="OLD">
          <idno type="IdRef">026404788</idno>
          <idno type="ISNI">0000 0001 2188 0914 </idno>
          <idno type="ROR">https://ror.org/0220k9r37</idno>
          <orgName>Université Paris Descartes - Paris 5</orgName>
          <orgName type="acronym">UPD5</orgName>
          <date type="start">1971-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>
            <address>
              <addrLine>12, rue de l'École de Médecine - 75270 Paris cedex 06</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.parisdescartes.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-341038" status="VALID">
          <idno type="IdRef">232435103</idno>
          <idno type="ISNI">000000010626358X</idno>
          <idno type="ROR">https://ror.org/02cte4b68</idno>
          <orgName>Institut de Chimie - CNRS Chimie</orgName>
          <orgName type="acronym">INC-CNRS</orgName>
          <date type="start">2009-10-01</date>
          <desc>
            <address>
              <addrLine>CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE3 rue Michel-Ange75794 PARIS CEDEX 16 - France</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.cnrs.fr/inc</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>