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                <forename type="first">Sabrina</forename>
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                <forename type="first">Jean</forename>
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            <idno type="halRefHtml">&lt;i&gt;Applied Biochemistry and Biotechnology&lt;/i&gt;, 2016, 179 (4), pp.671-683. &lt;a target="_blank" href="https://dx.doi.org/10.1007/s12010-016-2023-x"&gt;&amp;#x27E8;10.1007/s12010-016-2023-x&amp;#x27E9;&lt;/a&gt;</idno>
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                <title xml:lang="en">Olive Recombinant Hydroperoxide Lyase, an Efficient Biocatalyst for Synthesis of Green Leaf Volatiles</title>
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                    <forename type="first">Jean-Marie</forename>
                    <surname>Desjobert</surname>
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                <title level="j">Applied Biochemistry and Biotechnology</title>
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                  <biblScope unit="issue">4</biblScope>
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                  <date type="datePub">2016-02-18</date>
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                <term xml:lang="en">3Z-Hexenal</term>
                <term xml:lang="en">Hexanal</term>
                <term xml:lang="en">Bioconversion</term>
                <term xml:lang="en">Green note</term>
                <term xml:lang="en">C6-aldehydes</term>
                <term xml:lang="en">Hydroperoxide lyase</term>
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              <p>Volatile C6-aldehydes are the main contributors to the characteristic odor of plantsknown as Bgreen note^ and are widely used by the flavor industry. Biotechnological processeswere developed to fulfill the high demand in C6-aldehydes in natural flavorants and odorants.Recombinant hydroperoxide lyases (HPLs) constitute an interesting alternative to overcomedrawbacks arising from the use of HPL from plant extracts. Thus, olive recombinant 13-HPLwas assayed as biocatalysts to produce C6-aldehydes. Firstly, a cDNA encoding for olive HPLof Leccino variety was isolated and cloned in pQE-30 expression vector. In order to improvethe enzyme solubility, its chloroplast transit peptide was deleted. Both enzymes (HPL wild typeand HPL deleted) were expressed into Escherichia coli strain M15, purified, characterized, andthen used for bioconversion of 13-hydroperoxides of linoleic and linolenic acids. Aldehydesproduced were extracted, then identified and quantified using gas chromatography and massspectrometry. Recombinant HPL wild type (HPLwt) allowed producing 5.61 mM of hexanaland 4.39 mM of 3Z-hexenal, corresponding to high conversion yields of 93.5 and 73 %,respectively. Using HPL deleted (HPLdel) instead of HPLwt failed to obtain greater quantitiesof hexanal or 3Z-hexenal. No undesirable products were formed, and no isomerization of 3Zhexenalin 2E-hexenal occurred. The olive recombinant HPLwt appears to be a promisingefficient biocatalyst for the production of C6-aldehydes</p>
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