The innate immune response of equine bronchial epithelial cells is altered by training - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Veterinary Research Année : 2015

The innate immune response of equine bronchial epithelial cells is altered by training

Linda Frellstedt
  • Fonction : Auteur correspondant
  • PersonId : 979672

Connectez-vous pour contacter l'auteur
Philippe Gosset
Gwenola Kervoaze
  • Fonction : Auteur
  • PersonId : 979673
Aymeric Hans
Christophe Desmet
  • Fonction : Auteur
  • PersonId : 979674
Dimitri Pirottin
  • Fonction : Auteur
  • PersonId : 979675
Fabrice Bureau
  • Fonction : Auteur
  • PersonId : 979676
Pierre Lekeux
  • Fonction : Auteur
  • PersonId : 979677
Tatiana Art
  • Fonction : Auteur
  • PersonId : 979678

Résumé

AbstractRespiratory diseases, including inflammatory airway disease (IAD), viral and bacterial infections, are common problems in exercising horses. The airway epithelium constitutes a major physical barrier against airborne infections and plays an essential role in the lung innate immune response mainly through toll-like receptor (TLR) activation. The aim of this study was to develop a model for the culture of equine bronchial epithelial cells (EBEC) in vitro and to explore EBEC innate immune responses in trained horses. Bronchial epithelial biopsies were taken from 6 adult horses during lower airway endoscopy. EBEC were grown in vitro by an explant method. The innate immune response of EBEC was evaluated in vitro by treatment with TLR ligands. TLR3 is the most strongly expressed TLR at the mRNA level in EBEC and stimulation of EBEC with Poly(I:C), an analog of viral dsRNA, triggers a strong secretion of IFN-β, TNF-α, IL-6 and CXCL8. We further evaluated the EBEC innate immune response in horses that underwent a 4-month-training program. While training had no effect on TLR mRNA expression in EBEC as well as in bronchial biopsies, it increased the production of IFN-β after stimulation with a TLR3 ligand and decreased the secretion of TNF-α and IL-6 after stimulation with a TLR2 and TLR3 ligand. These findings may be implicated in the increased risk for viral and bacterial infections observed in sport horses. Altogether, we report a successful model for the culture of EBEC that can be applied to the investigation of pathophysiologic conditions in longitudinal studies.
Fichier principal
Vignette du fichier
13567_2014_Article_126.pdf (1.38 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Loading...

Dates et versions

hal-01290650 , version 1 (18-03-2016)

Identifiants

Citer

Linda Frellstedt, Philippe Gosset, Gwenola Kervoaze, Aymeric Hans, Christophe Desmet, et al.. The innate immune response of equine bronchial epithelial cells is altered by training. Veterinary Research, 2015, 46 (1), pp.3. ⟨10.1186/s13567-014-0126-3⟩. ⟨hal-01290650⟩

Collections

ARINRAE-VR ARINRAE
29 Consultations
48 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More